AIMS AND CONTENT

LEARNING OUTCOMES

Module “Diagnostic Biotechnologies”

To introduce students to the methods derived from basic biotechnologies that were transferred to medical diagnostics

To know the main analyses of clinical biochemistry and their interpretation to verify metabolism and function of different organs

Module “Nanotechnology”

The aim of the course is to give to the students an overview of the most recent and advanced nanobiotechnologies and nanobiomaterials. In particular, the two aspects of nanobioscience and nanobiotechnology application take place: structural proteomics and functional proteomics. In the first case, the novel method of protein nanocrystallography and the advanced synchrotron radiation techniques, including micro and nano-focussed high intense synchrotron radiation and the new trends in radiation damage reduction are presented. In the second case, the most innovative technologies of protein microarrays NAPPA (Nucleic Acid Programmable protein Array) and their fluorescent-label as well as label-free technology of are demonstrated. In the both cases the application to the regenerative medicine are underlined

TEACHING METHODS

Frontal lessons and laboratory.

SYLLABUS/CONTENT

Module “Diagnostic Biotechnologies”

Medical diagnostics based on biotech methods

1. Management systems of biotech industry and their use in medical diagnostics laboratories
2. Strategic marketing for health market, business plan, cash flow
3. Mass spectrometry in human diagnostics (Proteomics, Therapeutic drug monitoring)
4. High speed cell sorting, single cell sorting
5. Helth informatics, artificial intellingence, expert systems, computer assisted diagnosis
6. Basic and advance immunometry methods, reading techniques
7. Mission and vision of future medical diagnostics laboratories.

Clinical Biochemistry

Main laboratory test in clinical biochemistry

1. Blood: hemochromocytometric test; electrophoretic profile of serum proteins; plasma enzymes in diagnosis; non-protein nitrogen.
2. Hepatic function tests: enzymes (AST, ALT, GGT, AP), bilirubin, serum proteins.
3. Glucose metabolism test: hormones regulating glycemia; causes for hyper- and hypo-glycemia; diagnostic parameter for diabete mellitus; laboratory tests in diabetic ketoacidosis; glucose tolerance test; glycosylated hemoglobin; ketonic compounds.
4. Lipid metabolism test: lipoprotein and apolipoprotein classification and their metabolism; reference value for different classes of lipids; dyslipidemic phenotypes.
5. Urine analysis and renal function test: physical chemical urine analysis; renal function test (creatinine clearance, BUN-blood urea nitrogen, urine concentration capacity.
6. Endocrinology: hints to the function of the main hormones, basal levels and conditions of hyper-/hypo-secretion; laboratory tests in the different hyper-/hypo-secretion conditions.

Module “Nanotechnology”

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RECOMMENDED READING/BIBLIOGRAPHY

Module “Diagnostic Biotechnologies”

Handouts provided by the Professors

Module “Nanotechnology”

1. Pechkova E., Nicolini C., Proteomics and Nanocrystallography, Kluwer Academic Press, 1-216, 2003.
2. Nicolini C., Nanobiotechnology and Nanobiosciences, Pan Stanford Series on Nanobiotechnology Volume 1, pp. 1-367, London - New York - Singapore, 2009.
3. Biofisica e Propedeutica Biofisica, C. Nicolini, Aracne Editrice, Roma 2009 (ISBN 978-88-548-2373-0), Capitoli 3 (pp.158-170), Capitolo 6 (pp.385-395)
4. Functional Proteomics and Nanotechnology-based Microarrays, (Eds. Nicolini C., LaBaer J.) Pan Stanford Series on Nanobiotechnology, Volume 2, pp. 1-308, London - New York - Singapore, 2010.
5. Structural Proteomics and Synchrotron Radiation (Eds. Pechkova, E., Riekel, C.)  Pan Stanford Series on Nanobiotechnology Volume 3, pp. 1-447, London - New York - Singapore, 2012.
6. Nicolini C., Pechkova E. Aspetti Biochimici su Struttura e Funzione delle Biomolecole. In: Biochimica Umana Con Schede Cliniche. Editore: Francesco Salvatore. Capitolo 24, p. 775-798, Napoli: Idelson Gnocchi Edizioni Scientifiche

TEACHERS AND EXAM BOARD

Exam Board

SANTINA BRUZZONE (President)

CHIARA MARTA FRESIA

MICHELE MUSSAP

EVGENIYA PESHKOVA

LESSONS

LESSONS START

October 3, 2016

Class schedule

BIOTECHNOLOGY DIAGNOSTIC AND NANOTECHNOLOGY AND LABORATORY

EXAMS

EXAM DESCRIPTION

Oral.

ASSESSMENT METHODS

Oral examination

Exam schedule