The aim of the course is to provide students with theoretical knowledge but also practical on the assumptions and techniques underlying the engineering of proteins and antibodies. In addition, part of the course will be dedicated to the deepening of the techniques of chemical synthesis of proteins.
Regarding the form of Protein engineering, will initially be resumed and expanded some concettivi base relative to the production of recombinant proteins. Thus, different approaches will be analyzed in order to obtain modified proteins or optimized for different applications, the techniques used and the systems of selection / screening, and using examples from the literature or used for production / commercial.
In the section of the course on the engineering of antibodies will be given with the three-dimensional structure of immunoglobulins and will be addressed later, in the chronological order of creation, the different techniques of humanization and production of recombinant phage display antibody.
In the form of Chemical Engineering explain the main techniques for the chemical synthesis of peptides and proteins and applications resulting from these techniques.
Frontal lessons.
There are currently no adequate texts. In addition to lecture notes, students will have the educational materials in the form of handouts and publications from the scientific literature.
Ricevimento: The teacher receives by appointment at Biochemistry - DIMES, Viale Benedetto XV No. 1, first floor, Genoa. The talks may take place, by appointment, also via Skype For appointments please send an email to: tonetti@unige.it.
Ricevimento: By appointment by email: Enrico.Millo@unige.it
Ricevimento: The lecturer will be available for help by previous email appointment (fghiotto@unige.it)
MICHELA TONETTI (President)
FABIO GIUSEPPE GHIOTTO
ENRICO MILLO
March 1, 2017.
PROTEIN ENGINEERING
Oral.
The assessment of the achievement of educational objectives will be effected by oral exams. The date of the appeals will be agreed with the students.
