OVERVIEW

The course of Instrumentation in Genomics and Proteomics aims to provide students with the methodological and instrumental knowledge for the acquisition of theoretical-practical skills which are essential, up today, for basic and applied research in biochemistry and biotechnology.

AIMS AND CONTENT

LEARNING OUTCOMES

Instrumentation in genomics Overview of the most used technologies applied to genomic analysis both at the chromosomal and at the DNA sequence level. Instrumentation in proteomics The course aims to provide the basis for the understanding of the mass spectrometry techniques useful for proteins characterization. Chromatographic Techniques The unit provides an overview of the sampling procedures and chromatographic techniques used in proteomics.

AIMS AND LEARNING OUTCOMES

Attendance and participation in the planned educational activities (lectures and laboratory experiences) will enable the student to acquire the knowledge necessary for biotechnology research.

Specifically, the student will be able to understand, learn and describe the methodologies and instrumentation used in genomics and proteomics.

PREREQUISITES

Fundamental requirements for the study of the subject are the knowledge of general, inorganic, organic chemistry, biochemistry and molecular biology.
In particular, the student will have to know in detail the basic techniques in use in the biochemical and molecular biological field

TEACHING METHODS

The teaching consists of lectures completed by laboratory activities. Activities aimed at applying the knowledge acquired in the classroom during the lectures are foreseen.
Lectures in the classroom are delivered through the use of multimedia presentations and the constant use of the blackboard for the writing of molecular structures and the description of instruments and methods.

SYLLABUS/CONTENT

Instrumental analytical methods; choice of the analytical method and figures of merit; methods and parameters
chromatography; liquid chromatography; detectors for liquid chromatography.
Mass spectrometry techniques for the structural characterization of molecules of biological interest at different molecular weights. Principles and use of microarrays based systems. Technologies for DNA mutation research. DNA sequencing methods. Screening methods.

RECOMMENDED READING/BIBLIOGRAPHY

All slides used during the lessons and other teaching material will be available on AulaWeb before the beginning of the lessons together with other bibliographic material in pdf format.

The books indicated are suggested as a supporting text and are available and can be consulted together with other books for further information by the teacher.

Texts advised for further information: Ringe-Petsko- Structure and function of proteins- Zanichelli Mauro Maccarrone - Biochemical and biomolecular methods. Tools and techniques for the laboratory of the new millennium - Zanichelli Carmela Bonaccorsi Di Patti, Roberto Contestabile, Martino Luigi Di Salvo - Biochemical methods - Zanichelli

TEACHERS AND EXAM BOARD

Exam Board

GIANLUCA DAMONTE (President)

PAOLO MALATESTA (President)

PAOLA FRANCESCA RIVARO (President)

LESSONS

LESSONS START

The first semester lessons will start from the beginning of October 2019. The second semester lessons will start from the beginning of March 2020.

Class schedule

The timetable for this course is available here: Portale EasyAcademy