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CODE 64556
ACADEMIC YEAR 2016/2017
CREDITS
SCIENTIFIC DISCIPLINARY SECTOR BIO/11
LANGUAGE Italiano
TEACHING LOCATION
SEMESTER 2° Semester
PREREQUISITES
Propedeuticità in ingresso
Per sostenere l'esame di questo insegnamento è necessario aver sostenuto i seguenti esami:
  • Pharmaceutical chemistry and technology 8451 (coorte 2016/2017)
  • ANIMAL BIOLOGY AND HUMAN ANATOMY (I.C.)(PCT MD) 64555 2016
MODULES Questo insegnamento è un modulo di:

OVERVIEW

Molecular Biology is a module constituted by twelve lectures, for a total of 24 hours. 

AIMS AND CONTENT

LEARNING OUTCOMES

The module is aimed to give to the students a hint on the structure and the complexity of the eukaryotic genome and its functional organization compared to the prokaryotic genomes, covering evolutionary aspects. The module aims to clarify the mechanism controlling genome stability, including those responsible of replication accuracy and DNA-repair. The course covers with particular emphasis the mechanisms controlling gene expression both in prokaryotes and eukaryotes. The last part of the module covers the most important techniques for the analysis of nucleic acids and production of recombinant proteins.

TEACHING METHODS

Lectures

SYLLABUS/CONTENT

List of the 12 lectures

1.      Course overview, exams, suggested books. General introduction and basic concepts: biomolecules, biological reactions, enzymatic catalisis. The genetic code and the reading frames.

2.      Genes and genomes. Evolution of genomes. The structural organization of eukariotic geneome.

3.      DNA replication and its accuracy.

4.      DNA repair mechanisms

5.      Gene expression control in prokaryotes: promoters, repressors, attenuators and riboswitches.

6.      Gene expression control in eukaryotes: eukaryotic promoter, enhancers, insulators, LCR

7.      Gene expression control in eukaryotes: Chromatin and  epigenetic regulators.

8.      Gene expression control in eukaryotes: Post-transcriptional regulation. Alternative splicing.

9.      Nuclear transport, mRNA stability, non sense mediated decay, miRNAs and other non-coding RNA

10.   Recombinant DNA tecnologies. Libraries and strategy for their screening. Nucleic Acid hybridization and related techniques: Blotting, in situ, microarrays.

11.   Polimerase chain reaction and its use.

Sequencing and next generation sequencing.

RECOMMENDED READING/BIBLIOGRAPHY

L'essenziale di Biogia Moleclare della Cellula (Alberts, ed Zanichelli)

TEACHERS AND EXAM BOARD

Exam Board

PAOLO MALATESTA (President)

ANNA MARIA SCHITO (President)

RODOLFO QUARTO

LESSONS

Class schedule

The timetable for this course is available here: Portale EasyAcademy

EXAMS

Exam schedule

Data appello Orario Luogo Degree type Note
18/04/2017 09:00 GENOVA Scritto
14/06/2017 09:00 GENOVA Scritto
28/06/2017 09:00 GENOVA Scritto
13/07/2017 09:00 GENOVA Scritto
07/09/2017 09:00 GENOVA Scritto
22/09/2017 09:00 GENOVA Scritto
23/01/2018 09:00 GENOVA Scritto
06/02/2018 09:00 GENOVA Scritto