CODE 61785 ACADEMIC YEAR 2017/2018 CREDITS 6 cfu anno 1 BIOLOGIA MOLECOLARE E SANITARIA 9015 (LM-6) - SCIENTIFIC DISCIPLINARY SECTOR BIO/10 LANGUAGE Italian TEACHING LOCATION SEMESTER 1° Semester OVERVIEW This class is targeted to students that have already learned Biochemistry in the preceding three year course of Biology. Here we will analyze up-to-date molecular aspects of the relationship between protein structure and specific localization and functional roles. AIMS AND CONTENT AIMS AND LEARNING OUTCOMES This class concerns an up-to-date description of the mechanisms that regulate the connections between structure and function of different human polypeptides. It will be considered the link between the several processes involved in the control of protein structure and the problem of their dysregulation in human pathologies. We will discuss how specific structural signatures on different proteins can regulate their post-transductional modifications, domains organization, acquirement of the native conformation, interaction with molecular partners, localization into specific sub-cellular compartments or extra-cellular release, half-life of a protein. At the end of the class, each student can select a recent scientific paper, among several of them proposed by the teacher, pertaining to a topic of the syllabus. The student could present and discuss the results described in the relevant paper during the oral exam. Aim of this class is to stimulate the appeal of the student for this rapidly evolving discipline and to encourage his approach to the scientific research. TEACHING METHODS Teaching is performed by classroom-taught lessons with the aid of slides that are available to the students on the Aulaweb. SYLLABUS/CONTENT General aspects of the links among the structure, functions, compartmentation, traffic and turnover of proteins. Types and outcomes of reversible e irreversible post-transductional modifications of proteins, human pathologies related to errors in post-translational processes. Protein domains: modular structures and formation of protein complexes. The CATH data base. Mechanisms for the acquirement of higher structural levels by globular proteins and collagen. Folding and misfolding of proteins, the molecular chaperones. Activity of the protein disulfide isomerase, cyclofilins, heat shock proteins, transglutaminase. Human pathologies associated with specific protein misfolding. Signals involved in the cellular sorting of proteins: molecular bases for import and export in the endoplasmic reticulum, Golgi apparatus, mitochondria, peroxisomes, nucleus, lysosomes.Patological effects of errors in the protein sorting. Signal sequences on organular proteins: their effects on endocytosis, secretion, non secretory exports and damages related to their deficit. Coated vesicles, GTP binding proteins and SNARE. Intra and extracellular proteolysis: ubiquitin and calcium-dependent systems, caspases and metallo proteases. Lysosomal protein degradation and lysosomal storage diseases. Short and long term responses to insulin and their regulation. The Notch system and the concept of receiver and sender cells. RECOMMENDED READING/BIBLIOGRAPHY -Alberts-Molecular Biology of the cells- Garland Science Ed. TEACHERS AND EXAM BOARD BIANCA SPARATORE MONICA AVERNA Ricevimento: Students can meet the teachers by booking individual appointments. The teachers can be contacted either by e-mail or telephone. e mail: monica.averna@unige.it phone: 010 3538423 Exam Board MONICA AVERNA (President) BIANCA SPARATORE (President) LESSONS Class schedule BIOCHEMISTRY OF CELL SIGNALING EXAMS Exam schedule Data appello Orario Luogo Degree type Note 29/01/2018 09:30 GENOVA Orale 16/02/2018 09:30 GENOVA Orale 15/06/2018 09:30 GENOVA Orale 29/06/2018 09:30 GENOVA Orale 13/07/2018 09:30 GENOVA Orale 27/07/2018 09:30 GENOVA Orale 17/09/2018 09:30 GENOVA Orale