CODE 91081 ACADEMIC YEAR 2018/2019 CREDITS 4 cfu anno 2 BIOLOGIA MOLECOLARE E SANITARIA 9015 (LM-6) - GENOVA SCIENTIFIC DISCIPLINARY SECTOR BIO/10 LANGUAGE English TEACHING LOCATION GENOVA SEMESTER 2° Semester TEACHING MATERIALS AULAWEB OVERVIEW The course consist of two modules: Biochemistry II and Structural Biology. The Module of Structural Biology is aimed at students in biotechnology, biology, chemistry and materials science. AIMS AND CONTENT LEARNING OUTCOMES "The Structural Biology course aims to provide the basis for understanding the three-dimensional structure of macromolecules of biochemical and biotechnological interest such as proteins and nucleic acids. The functional aspects to structural ones will be discussed through various examples present in the literature." TEACHING METHODS Frontal lessons. Laboratory exercises SYLLABUS/CONTENT Chemical bonds that stabilize polypeptides: covalent bonds (peptide bond and its properties), hydrogen bonds, salt bridges, hydrophobic bonds, and weak electrostatic interactions. Molecular structures, and their stability. Main classes of organic organic molecules and basis of nucleic acids and proteins structures. Structure of nucleic acids. Primary and secondary structure of DNA and RNA and their integration into the genome. Tertiary structure of tRNA and rRNA. Methods for the definition of the primary structure of nucleic acids. Quaternary structure of the ribosome (rRNA protein integration). Structure of human chromosomes. Protein structure and function. Methods for studing the primary protein structure; Edman degradation, electrospray mass spectrometry, and single or multiple analyzers (MS / MS) MALDI. Levels of structural organization of proteins (primary, secondary, tertiary and quaternary structures). Motifs and domains. Dynamic and thermodynamic folding processes. Molecular evolution, conservation of the three-dimensional fold (divergent evolution, convergent evolution) and protein topologies. Classification of protein structure in structural hierarchies with increasing detail. Correlation of structures with the biological activity of proteins (examples of literature). Principles of crystallization and analysis of crystals of biological macromolecules. Hydrodynamic principles and models for the study of low resolution proteins. Protein Representative Graphing Methods: Lesk & Hardman, RIBBON, TOPS. Van der Waals surfaces. Protein surfaces and interactions with other proteins, ligands and nucleic acids. Criteria for the recognition of binding sites and catalysis. Catalysis and enzymatic kinetics: mechanisms of action of some classes of enzymes of biotechnological and biomedical interest. RECOMMENDED READING/BIBLIOGRAPHY Protein structure and function - Petsko G. A.; Ringe D. – Zanichelli Introduction to Protein Structure - Branden C., Tooze J. - Garland Pub TEACHERS AND EXAM BOARD GIANLUCA DAMONTE Ricevimento: By appointment by email: gianluca.damonte@unige.it Exam Board GIANLUCA DAMONTE (President) ANNALISA SALIS LESSONS LESSONS START March 1st, 2018 Class schedule The timetable for this course is available here: Portale EasyAcademy EXAMS Exam schedule Data appello Orario Luogo Degree type Note 22/01/2019 09:00 GENOVA Orale 01/02/2019 09:00 GENOVA Orale 21/02/2019 09:00 GENOVA Orale 17/06/2019 09:00 GENOVA Orale 04/07/2019 09:00 GENOVA Orale 18/07/2019 09:00 GENOVA Orale 19/09/2019 09:00 GENOVA Orale