CODE 98812 ACADEMIC YEAR 2021/2022 CREDITS 2.5 cfu anno 2 MEDICAL-PHARMACEUTICAL BIOTECHNOLOGY 10598 (LM-9) - GENOVA SCIENTIFIC DISCIPLINARY SECTOR BIO/10 LANGUAGE English TEACHING LOCATION GENOVA SEMESTER 2° Semester TEACHING MATERIALS AULAWEB OVERVIEW The course of Instrumentation in Genomics and Proteomics aims to provide students with the methodological and instrumental knowledge for the acquisition of theoretical-practical skills which are essential, up today, for basic and applied research in biochemistry and biotechnology. AIMS AND CONTENT LEARNING OUTCOMES Instrumentation in genomics Overview of the most used technologies applied to genomic analysis both at the chromosomal and at the DNA sequence level. Instrumentation in proteomics The course aims to provide the basis for the understanding of the mass spectrometry techniques useful for proteins characterization. Chromatographic Techniques The unit provides an overview of the sampling procedures and chromatographic techniques used in proteomics. AIMS AND LEARNING OUTCOMES Attendance and participation in the planned educational activities (lectures and laboratory experiences) will enable the student to acquire the knowledge necessary for biotechnology research. Specifically, the student will be able to understand, learn and describe the methodologies and instrumentation used in genomics and proteomics. PREREQUISITES Fundamental requirements for the study of the subject are the knowledge of general, inorganic, organic chemistry, biochemistry and molecular biology. In particular, the student will have to know in detail the basic techniques in use in the biochemical and molecular biological field TEACHING METHODS The teaching consists of lectures completed by laboratory activities. Activities aimed at applying the knowledge acquired in the classroom during the lectures are foreseen. Lectures in the classroom are delivered through the use of multimedia presentations and the constant use of the blackboard for the writing of molecular structures and the description of instruments and methods. SYLLABUS/CONTENT Instrumental analytical methods; choice of the analytical method and figures of merit; methods and parameters chromatography; liquid chromatography; detectors for liquid chromatography. Mass spectrometry techniques for the structural characterization of molecules of biological interest at different molecular weights. Principles and use of microarrays based systems. Technologies for DNA mutation research. DNA sequencing methods. Screening methods. RECOMMENDED READING/BIBLIOGRAPHY All slides used during the lessons and other teaching material will be available on AulaWeb before the beginning of the lessons together with other bibliographic material in pdf format. The books indicated are suggested as a supporting text and are available and can be consulted together with other books for further information by the teacher. Texts advised for further information: Ringe-Petsko- Structure and function of proteins- Zanichelli Mauro Maccarrone - Biochemical and biomolecular methods. Tools and techniques for the laboratory of the new millennium - Zanichelli Carmela Bonaccorsi Di Patti, Roberto Contestabile, Martino Luigi Di Salvo - Biochemical methods - Zanichelli TEACHERS AND EXAM BOARD GIANLUCA DAMONTE Ricevimento: GIANLUCA DAMONTE: By appointment by email: gianluca.damonte@unige.it PAOLO MALATESTA Ricevimento: Tuesday h. 12:00. Exam Board GIANLUCA DAMONTE (President) PAOLO MALATESTA (President) LESSONS LESSONS START The first semester lessons will start from the beginning of October 2019. The second semester lessons will start from the beginning of March 2021. Class schedule The timetable for this course is available here: Portale EasyAcademy EXAMS EXAM DESCRIPTION The oral exam lasts about 30 minutes / student on the topics covered in class. The student is asked three questions of increasing difficulty aimed at assessing the degree of study of the subject and preparation of the student. ASSESSMENT METHODS Details on how to prepare for the exam and the degree of detail required for each topic will be provided at the beginning of the course and confirmed during lectures and exercises. The oral exam will focus on the topics covered during the lectures and will aim to assess the achievement of the appropriate level of knowledge, the ability to use the theoretical notions to integrate the different concepts addressed in class and developed in the laboratory. The ability to present the topics clearly and with correct terminology will also be evaluated. FURTHER INFORMATION Attendance at lectures is mandatory.
