CODE 67405 ACADEMIC YEAR 2022/2023 CREDITS 3 cfu anno 4 8745 (LM-41) - GENOVA SCIENTIFIC DISCIPLINARY SECTOR MED/07 LANGUAGE Italian TEACHING LOCATION GENOVA SEMESTER 1° Semester MODULES Questo insegnamento è un modulo di: INFECTIOUS DISEASES AND CLINICAL MICROBIOLOGY TEACHING MATERIALS AULAWEB OVERVIEW Microbiology is a science that studies microorganisms, microscopic organisms having an extraordinary impact on the entire life and the composition of our planet. Microorganisms and humans have a very close relationship. Upon the completion of this course the students should know the biology of the microorganisms and their strategies to cause diseases in humans. AIMS AND CONTENT AIMS AND LEARNING OUTCOMES Objectives: General Microbiology: to know the characteristics of microorganisms useful to isolate them from clinical samples in the laboratory and the microbiological bases of antibiotic use. Clinical microbiology: to know the basic principles in the direct and indirect microbiology diagnosis of infectious diseases Clinical microbiology: to know the microbiological diagnostic flow-charts that can be used for the diagnosis of respiratory tract (high and low tracts), uro-genital tract, gastro-enteric tract, central nervous system, cardiovascular system infections and sepsis. Clinical microbiology: to know the microbiological diagnostic flow-charts that can be used for opportunistic infections in the immunocompromised patient TEACHING METHODS The teaching methods include: lectures (30 total hours) SYLLABUS/CONTENT Differential characteristics for the identification of the following microorganisms: Staphylococci, Streptococci, Pneumococci, Enterococci, Mycobacteria, Neisseria, Hemophiles, Moraxella, atypical germs, Enterobacteria, Pseudomonas, Acinetobacter, Clostridia, Fungi (candida and aspergillus), Herpesvirus, HIV, Hepatitis, Paramyxovirus, HPV. Microbiological diagnosis (direct-indirect diagnosis, executive protocols, microscopic examination, culture, antibiogram, molecular microbiological techniques, ELISA, IF Microbiological diagnosis of respiratory, uro-genital, cardio-circulatory infections (blood culture and endotoxin test), gastro-enteric, central nervous system Microbiological diagnosis of HIV and opportunistic infections in the immunocompromised patient RECOMMENDED READING/BIBLIOGRAPHY Microbiologia Clinica a cura di E.A. Debbia. Casa Editrice Esculapio TEACHERS AND EXAM BOARD ANNA MARCHESE Ricevimento: An appointment, fixed by an electronic request at the address: email@example.com, is needed Exam Board ANDREA DE MARIA (President) MALGORZATA KAROLINA MIKULSKA (President) ANTONIO DI BIAGIO DANIELE ROBERTO GIACOBBE ANNA MARCHESE ANTONIO VENA MATTEO BASSETTI (President and Coordinator of Integrated Course) LESSONS Class schedule L'orario di tutti gli insegnamenti è consultabile all'indirizzo EasyAcademy. EXAMS EXAM DESCRIPTION Oral examination ASSESSMENT METHODS The evaluation is carried out through the only final exam which seeks to ascertain the actual acquisition by the student of the expected learning results. To demonstrate their knowledge, the students will be able to know: -the main characteristics of prokaryotes, eukaryotes and viruses and their classification. The host-microbe interactions (symbiosis, parasitism, opportunism, colonization, infection, disease); -the characteristics of bacteria (bacterial structure, growth and microbial metabolism, overview of bacterial genetics); -the bacterial pathogenicity and virulence factors (input, adherence to host tissue, invasion, permanence, exotoxins and endotoxins, hydrolytic enzymes, antiphagocytic mechanisms), intracellular parasites and heterogeneity antigen; -the major classes of chemotherapeutic agents and antibiotics (beta-lactams, glycopeptides, aminoglycosides, tetracyclines, quinolones, chloramphenicol, macrolides, clindamycin, oxazolidinones, streptogramins, nitroimidazoles, rifampin) and their main characteristics (spectrum of action, target, pharmacokinetics and mechanisms of drug resistance); -susceptibility and antimicrobial resistance and quantitative and qualitative determination of antibiotic activity; -the general characteristics of the viruses (structure and composition, viral classification, viral replication, viral genetics overview); -the mechanism of viral penetration into the host, viral transmission, spreading, tropism, virulence and cytopathic effects, persistence, latency, reactivation); -the antiviral drugs and resistances; -the general characteristics of pathogenic fungi and parasites. A minimum mark of 18/30 is required to pass the module. The mark 30/30 with any honors will correspond to an excellent knowledge of all argoments.