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INSTRUMENTATION FOR GENOMICS AND PROTEOMICS

CODE 98812
ACADEMIC YEAR 2022/2023
CREDITS
  • 2.5 cfu during the 2nd year of 10598 MEDICAL-PHARMACEUTICAL BIOTECHNOLOGY(LM-9) - GENOVA
  • SCIENTIFIC DISCIPLINARY SECTOR BIO/10
    LANGUAGE English
    TEACHING LOCATION
  • GENOVA
  • SEMESTER 2° Semester
    TEACHING MATERIALS AULAWEB

    OVERVIEW

    The course of Instrumentation in Genomics and Proteomics aims to provide students with the methodological and instrumental knowledge for the acquisition of theoretical-practical skills which are essential, up today, for basic and applied research in biochemistry and biotechnology.

    AIMS AND CONTENT

    LEARNING OUTCOMES

    Instrumentation in genomics Overview of the most used technologies applied to genomic analysis both at the chromosomal and at the DNA sequence level. Instrumentation in proteomics The course aims to provide the basis for the understanding of the mass spectrometry techniques useful for proteins characterization. Chromatographic Techniques The unit provides an overview of the sampling procedures and chromatographic techniques used in proteomics.

    AIMS AND LEARNING OUTCOMES

    Attendance and participation in the planned educational activities (lectures and laboratory experiences) will enable the student to acquire the knowledge necessary for biotechnology research.

    Specifically, the student will be able to understand, learn and describe the methodologies and instrumentation used in genomics and proteomics.

    PREREQUISITES

    Fundamental requirements for the study of the subject are the knowledge of general, inorganic, organic chemistry, biochemistry and molecular biology.
    In particular, the student will have to know in detail the basic techniques in use in the biochemical and molecular biological field

    TEACHING METHODS


    The teaching consists of lectures completed by laboratory activities. Activities aimed at applying the knowledge acquired in the classroom during the lectures are foreseen.
    Lectures in the classroom are delivered through the use of multimedia presentations and the constant use of the blackboard for the writing of molecular structures and the description of instruments and methods.

    SYLLABUS/CONTENT

    Instrumental analytical methods; choice of the analytical method and figures of merit; methods and parameters
    chromatography; liquid chromatography; detectors for liquid chromatography.
    Mass spectrometry techniques for the structural characterization of molecules of biological interest at different molecular weights. Principles and use of microarrays based systems. Technologies for DNA mutation research. DNA sequencing methods. Screening methods.

    RECOMMENDED READING/BIBLIOGRAPHY

    All slides used during the lessons and other teaching material will be available on AulaWeb before the beginning of the lessons together with other bibliographic material in pdf format.

    The books indicated are suggested as a supporting text and are available and can be consulted together with other books for further information by the teacher.

    Texts advised for further information: Ringe-Petsko- Structure and function of proteins- Zanichelli Mauro Maccarrone - Biochemical and biomolecular methods. Tools and techniques for the laboratory of the new millennium - Zanichelli Carmela Bonaccorsi Di Patti, Roberto Contestabile, Martino Luigi Di Salvo - Biochemical methods - Zanichelli

    TEACHERS AND EXAM BOARD

    Exam Board

    GIANLUCA DAMONTE (President)

    PAOLO MALATESTA (President)

    LESSONS

    LESSONS START

    The first semester lessons will start from the beginning of October 2019. The second semester lessons will start from the beginning of March 2021.

    Class schedule

    All class schedules are posted on the EasyAcademy portal.

    EXAMS

    EXAM DESCRIPTION

    The oral exam lasts about 30 minutes / student on the topics covered in class.
    The student is asked three questions of increasing difficulty aimed at assessing the degree of study of the subject and preparation of the student.

    ASSESSMENT METHODS

    Details on how to prepare for the exam and the degree of detail required for each topic will be provided at the beginning of the course and confirmed during lectures and exercises.

    The oral exam will focus on the topics covered during the lectures and will aim to assess the achievement of the appropriate level of knowledge, the ability to use the theoretical notions to integrate the different concepts addressed in class and developed in the laboratory. The ability to present the topics clearly and with correct terminology will also be evaluated.

    FURTHER INFORMATION

    Attendance at lectures is  mandatory.