LEARNING OUTCOMES

The aim of the course is to provide students with the essential informations necessaries to carry out experiments on laboratory animals. Particularly, wide information on different animal models and their usefulness are given. Additionally, all necessary indications on law dispositions about animal research and on animal welfare are specified. Furthermore, the students will learn all methodologies currently in use to create mutant animals, including new scientific approaches. Specific examples are illustrated.

AIMS AND LEARNING OUTCOMES

• AIMS

Lessons aim to introduce students to the basic and most relevant aspects of preclinical and experimental research by means of specific animal models in biotechnology. Furthermore, the students will learn all methodologies currently in use to create mutant animals, including new scientific approaches. Specific examples will be illustrated and discussed.

• LEARNING OUTCOMES (DETAILED)

Students are required to retain knowledge of molecular and functional mechanisms that allow the design and creation of animal models for experimental research. Students must also know, comprehend and be able to compare the different models, their phenotypic expression and the relevance such models may offer; they will also be required to critically evaluate the possible effects of alterations of main cellular functions and their biological consequences. Students will also be expected to retain and use the proper scientific terminology and to be aware of all the necessary requirements and national and international law dispositions to perform animal research in biotech research.

PREREQUISITES

Students are required to retain previous knowledge on relevant structural and functional aspects in cell and molecular biology, with particular attention towards the regulation and modulation of gene expression. Good level of English is required as well to actively contribute to discussion and interaction  during classes and the final exam.

TEACHING METHODS

Classes will be managed as frontal lectures with Power Point presentations; during classes, students will be engaged in active discussion, via examples and specific exercises. If necessary, lessons may be provided on-line, through specific platforms and pre-arrangements made between the teacher, the students and the different Schools in the region. Students can take advantage of online material (lectures power point notes) available on the AulaWeb website; Lecturer(s) can be reached via email to arrange specific one-to-one meeting for further assistance/explanation as needed

SYLLABUS/CONTENT

Introduction to animal research principles for experimental and preclinical research; invertebrate models, zebrafish and amphibians; phenotyping; small and big mammalian models for preclinical research and specific techniques for in vivo disease-mimicking; national and international regulation for animal research: the “3Rs Principle” and the authorization process required to perform animal research; design and implementation of transgenic animal models for therapeutic approaches and behavioral disease.

RECOMMENDED READING/BIBLIOGRAPHY

Power point notes of class lectures are available for students to be downloaded from AulaWeb. Files are strictly for personal use only; files must not be shared on social media, posted on any internet website or put on any sharing platform; files must not be printed out for sale or photocopied.