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CODE 64556
ACADEMIC YEAR 2024/2025
CREDITS
SCIENTIFIC DISCIPLINARY SECTOR BIO/11
TEACHING LOCATION
  • GENOVA
SEMESTER 2° Semester
PREREQUISITES
Propedeuticità in ingresso
Per sostenere l'esame di questo insegnamento è necessario aver sostenuto i seguenti esami:
MODULES Questo insegnamento è un modulo di:

AIMS AND CONTENT

AIMS AND LEARNING OUTCOMES

The course aims to impart knowledge on the structure and complexity of the eukaryotic genome, its structural and functional organization. Additionally, it seeks to clarify the mechanisms responsible for genome stability, such as replication accuracy and DNA repair mechanisms. Special emphasis is placed on the regulation of gene expression in both bacteria and eukaryotes. Finally, the module provides an overview of molecular biology techniques relevant to the production of biotechnological drugs. The course is conducted interactively, aiming to engage students in theoretical experiments and encourage them to develop hypotheses and potential experimental strategies to test them.

TEACHING METHODS

Lectures are organized to actively engage participants in problem-solving activities, aimed at applying the knowledge acquired.

SYLLABUS/CONTENT

The 24-hour module is an intensive course aimed at providing, in three CFU (Crediti Formativi Universitari), fundamental knowledge of contemporary Molecular Biology and an overview of new techniques relevant to the production of biotechnological drugs. Specifically, the topics covered will be:

  1. Recap of basic concepts: biomolecules, biological reactions, enzymatic catalysis. The genetic code and reading frames.
  2. Genes and Genomes, evolution of genomes. Composition and organization of the eukaryotic genome.
  3. DNA replication and its accuracy. DNA damage repair mechanisms.
  4. Gene expression regulation in prokaryotes: promoters, repressors, attenuators, and riboswitches.
  5. Gene expression regulation in eukaryotes: eukaryotic promoter, enhancers, insulators, LCRs. Chromatin management and epigenetic information.
  6. Gene expression regulation in eukaryotes: post-transcriptional events. Alternative splicing. mRNA nuclear-cytoplasmic transport.
  7. mRNA stability. microRNAs.
  8. Translation and mechanisms ensuring accuracy. Aminoacyl-tRNA synthetases, GTPase control.
  9. Recombinant DNA techniques. Genomic libraries and screening strategies.
  10. DNA hybridization techniques.
  11. PCR (Polymerase Chain Reaction).
  12. DNA sequencing. Sanger method, automatic sequencers, and next-generation sequencing.

RECOMMENDED READING/BIBLIOGRAPHY

Bruce Alberts et al. L'essenziale di biologia molecolare della cellula (5° edizione)

Bruce Alberts et al. Biologia molecolare della cellula (6° edizione)

Watson J. Biologia molecolare del gene (8° edizione)

TEACHERS AND EXAM BOARD

LESSONS

Class schedule

The timetable for this course is available here: Portale EasyAcademy

EXAMS

EXAM DESCRIPTION

Oral Exam (about 30/40 minutes)

ASSESSMENT METHODS

The exam for the Molecular Biology module is designed to assess not only students' ability to memorize and repeat information, but primarily their capacity to apply acquired knowledge critically and constructively. Questions will encompass the entire course curriculum, focusing on the ability to organize answers logically and coherently, and to effectively apply learned knowledge to solve new problems. Emphasis will be placed on critical analysis, depth of individual study, mastery of concepts, and the ability to connect them to other knowledge gained throughout the course.

Agenda 2030 - Sustainable Development Goals

Agenda 2030 - Sustainable Development Goals
No poverty
No poverty
Good health and well being
Good health and well being
Quality education
Quality education
Gender equality
Gender equality
Peace, justice and strong institutions
Peace, justice and strong institutions
Partnerships for the goals
Partnerships for the goals