OVERVIEW

The course deals with (i) the pre-treatment of complex matrices containing bioactive compounds, (ii) and the quantitative determination of substances of pharmaceutical interest. It is developed with theoretical and practical lessons. The theoretical ones are preliminary to the practical ones and to the whole course comprehension. The main topics of the course are listed as follows.

AIMS AND CONTENT

LEARNING OUTCOMES

The course deals with the pre-treatment, extractive techniques and purification methods of bioactive molecules and substances of pharmaceutical interest. Instrumental and classical quantitative determination methods will be deepened, such as titration analysis. This course is developed with theoretical and practical lessons.

AIMS AND LEARNING OUTCOMES

The course allows to acquire theoretical and practical information regarding the main methods of sample pretreatment and purification. These methods include chromatography and instrumental analysis such as gas chromatography and HPLC. Specific theoretical and practical information will be acquired in terms of quantitative analysis of bioactive compounds, such as volumetric analysis, in particular in the areas of acid-base, complexometric, redox and precipitation titrations. The laboratory activities concern sample pre-treatment and purification techniques and the dosage of drugs.

PREREQUISITES

Inorganic chemistry.

TEACHING METHODS

The course is developed with a theoretical and practical lessons (96 hours for each student) concerning the pre-treatment and the quantitative dosage of substances of pharmaceutical interest, using classical and innovative methods.

Any Student with documented Specific Learning Disorders (SLD), or with any special needs, shall reach out to the Lecturer(s) and to the dedicated SLD Representative in the Department ( Prof. Luca Raiteri, Luca.Raiteri@unige.it ) before class begins, in order to liaise and arrange the specific learning methods and ensure proper achievement of the learning aims and outcomes. VERY IMPORTANT: any request for compensatory tools and adaptations in the exam MUST be done within 10 working days before the date of the exam according to the instructions that can be found at https://unige.it/disabilita-dsa/comunicazioni

SYLLABUS/CONTENT

• Guidelines on laboratory safety and chemical use.

• Introduction to the quantitative determination of compounds registered in FU XII Ed. and FE VIII Ed.

• Classification and selection of analitycal methods. Chemical, apparatus and methods for sample purification and for quantitative determination of compounds with medicinal uses.
• Calculation used in pharmaceutical analysis. Sampling, standardization and calibration.

• Techniques of preparing sample: filtration, extraction, distillation, chromatography. Gas chromatography and HPLC.
• Sample characterization: melting point, boiling point and refractive index determination.

• Introduction to volumetric titrations in pharmaceutical analysis: chemical equilibria in volumetric analysis. Preparation of standard solutions.

• Quantitative determination and applications: neutralization titrations, complexation and precipitation reactions and titrations, oxidation/reduction titrations, Diazotization Titration.

• Practical trainings

  Extraction with solvent, distillation in vacuo. TLC.

  Chromatographyc separation and analysis of a mixture of drugs. Quantitative determination of drugs by mean of neutralization titrations, complexation and precipitation reactions and titrations, oxidation/reduction titrations.

RECOMMENDED READING/BIBLIOGRAPHY

• Principi di Analisi Farmaceutica, Quarta Edizione - V. Cavrini e V. Andrisano, ESCULAPIO
• Analisi Farmaceutica David G. Watson Edra 2014
• Fondamenti di chimica analitica di Skoog e West, Terza Edizione - F. James Holler e Stanley R. Crouch, EDISES – ISBN 9788879598651
• Analisi chimico farmaceutica - F. Savelli e O. Bruno - PICCIN

TEACHERS AND EXAM BOARD

Exam Board

ELENA CICHERO (President)

FRANCESCA MICHELA MUSUMECI

ANNA CARBONE (Substitute)

PAOLA FOSSA (Substitute)

MICHELE TONELLI (Substitute)

LESSONS

LESSONS START

Second semester. Please see, EasyAcademy.

Class schedule

The timetable for this course is available here: Portale EasyAcademy

EXAMS

EXAM DESCRIPTION

Written prior to oral test. The first one is about mixture separation and quantitative determination of one proposed bioactive compound. The oral test will cover all the course main topics.

ASSESSMENT METHODS

The examination includes one written test and the oral test together with the evaluation of the experimental ability achieved by the student while attending to practical trainings in laboratory.

Exam schedule