PATHOLOGICAL ANATOMY - immunohistochemical techniques
Overview of immunohistochemical techniques
Preparatory treatment of the material for immuno-histochemical examinations
Basic immunohistochemical techniques (manual, semi-automatic and automatic)
Main markers in immuno-histochemistry (reading data-sheet, optimal dilution of new antibody, etc.)
Use of positive and negative controls
Preparation of pads
Maintenance and management tools
Waste disposal
Immuno-histochemistry on cytological material
Preparation prepared for extemporaneous diagnostics - Elective colors for intraoperative examinations - Preparation of cytology for intraoperative examination
PATHOLOGICAL ANATOMY - immunocytochemical techniques
Overview of immunocytochemical techniques
Acceptance and preparation of samples for immuno-cytochemical examinations
Cell separation on density gradient
Inclusion techniques for cytological material
Basic immunocytochemical techniques (manual, semi-automatic and automatic)
Immuno-cytochemical markers (data-sheet reading, optimal new antibody dilution, etc.)
Use of collective and individual PPE
Notes on biobanks
MOLECULAR BIOLOGY TECHNIQUES
Organization of behavioral areas and norms in the molecular biology laboratory
Acceptance of storage and conservation of the biological sample, work programs
Nucleic acid extraction with manual and automatic methods used in the laboratory
Dosing and evaluation of nucleic acids
PCR techniques,
Electrophoresis gel
Sequencing
Interpretation results
Maintenance and use of laboratory instruments
CQI and VEQ quality controls
Flow cytometry
Knowledge of the physical and technological bases of flow cytometry:
• General diagram of a flow cytometer (fluidics, optics, electronics)
• Meaning of the cellular physical parameters evaluated in cytometry
• Fluorescence signals
• Description of fluorine chromium
• Compensation operation
• Meaning of multiparametric analysis
• Gating strategy
• Data representation
Direct and indirect staining techniques of biological samples
Main application methodologies for the study of phenotype and lymphocyte functions, such as cytokine production and proliferation
HPLC and LC-MS / MS
Demonstration and characteristics of the operation of the modules of an HPLC
Extraction of a sample on a resin column
The 24 h urine collection is acidified for specific chromatographic examinations
Use of the S.I. in different methods
Reading of a chromatogram and possible changes on the retention times of the peaks
Intervention on the reading program to highlight and recognize the various operations so that a substance is recognized in the analytical phase
Exercise on the various methods in the elution phase of a sample
Procedure for stabilizing a column and identifying the various columns on an HPLC module
Demonstration and operation of an LC-MS / MS
Extraction of a sample for analysis in LC-MS / MS
Evaluation of an analysis calibration curve in LC-MS / MS
IMMUNOHEMATOLOGY
Blood group on adults
Blood group + TCD on newborns
Rh phenotype execution
Performing weak "D" test test
Direct / fractional Coombs test
Indirect Coombs test
Research and identification of anti-erythrocyte antibodies
Elution of autoantibodies
Automation in immunohematology;
Role and activity of a TSLB in a SIT
Hematovigilance and safety
Adverse reactions
Cross-breed erythrocyte match
Learning activities of specialized laboratory techniques with mandatory attendance of 100% of the planned hours of the laboratories and direct learning through the use of instruments with the guidance of professionals belonging to the same Professional Profile. There are no individual study hours.
The program has been explained in the heading "EDUCATIONAL OBJECTIVES (DETAIL)"
SONIA SCHIAVO (President)
CRISTINA CUSATO
ALESSANDRA SIGNORETTI (President and Coordinator of Integrated Course)
The practical training course begins after the end of the 1st semester lessons from the month of January to the month of February and after the lessons of the second semester from the month of April-May to the month of September with a break in the month of August
Verification of the ability to perform laboratory tests with description of the procedures for carrying out and the purposes of the test for clinical-diagnostic tests.
The exam takes place at the end of the completion of the annual internship. The student must demonstrate that he has achieved the intended objectives.
