AIMS AND CONTENT

AIMS AND LEARNING OUTCOMES

PATHOLOGICAL ANATOMY - immunohistochemical techniques

Overview of immunohistochemical techniques

Preparatory treatment of the material for immuno-histochemical examinations

Basic immunohistochemical techniques (manual, semi-automatic and automatic)

Main markers in immuno-histochemistry (reading data-sheet, optimal dilution of new antibody, etc.)

Use of positive and negative controls

Preparation of pads

Maintenance and management tools

Waste disposal

Immuno-histochemistry on cytological material

Preparation prepared for extemporaneous diagnostics - Elective colors for intraoperative examinations - Preparation of cytology for intraoperative examination

PATHOLOGICAL ANATOMY - immunocytochemical techniques

Overview of immunocytochemical techniques

Acceptance and preparation of samples for immuno-cytochemical examinations

Cell separation on density gradient

Inclusion techniques for cytological material

Basic immunocytochemical techniques (manual, semi-automatic and automatic)

Immuno-cytochemical markers (data-sheet reading, optimal new antibody dilution, etc.)

Use of positive and negative controls

Preparation of pads

Maintenance and management tools

Use of collective and individual PPE

Notes on biobanks

Waste disposal

MOLECULAR BIOLOGY TECHNIQUES

Organization of behavioral areas and norms in the molecular biology laboratory

Acceptance of storage and conservation of the biological sample, work programs

Nucleic acid extraction with manual and automatic methods used in the laboratory

Dosing and evaluation of nucleic acids

PCR techniques,

Electrophoresis gel

Sequencing

Interpretation results

Maintenance and use of laboratory instruments

CQI and VEQ quality controls

Flow cytometry

Knowledge of the physical and technological bases of flow cytometry:

• General diagram of a flow cytometer (fluidics, optics, electronics)

• Meaning of the cellular physical parameters evaluated in cytometry

• Fluorescence signals

• Description of fluorine chromium

• Compensation operation

• Meaning of multiparametric analysis

• Gating strategy

• Data representation

Direct and indirect staining techniques of biological samples

Main application methodologies for the study of phenotype and lymphocyte functions, such as cytokine production and proliferation

HPLC and LC-MS / MS

Demonstration and characteristics of the operation of the modules of an HPLC

Extraction of a sample on a resin column

The 24 h urine collection is acidified for specific chromatographic examinations

Use of the S.I. in different methods

Reading of a chromatogram and possible changes on the retention times of the peaks

Intervention on the reading program to highlight and recognize the various operations so that a substance is recognized in the analytical phase

Exercise on the various methods in the elution phase of a sample

Procedure for stabilizing a column and identifying the various columns on an HPLC module

Demonstration and operation of an LC-MS / MS

Extraction of a sample for analysis in LC-MS / MS

Evaluation of an analysis calibration curve in LC-MS / MS

IMMUNOHEMATOLOGY

Blood group on adults

Blood group + TCD on newborns

Rh phenotype execution

Performing weak "D" test test

Direct / fractional Coombs test

Indirect Coombs test

Research and identification of anti-erythrocyte antibodies

Elution of autoantibodies

Automation in immunohematology;

Role and activity of a TSLB in a SIT

Hematovigilance and safety

Adverse reactions

Cross-breed erythrocyte match

TEACHING METHODS

Learning activities of specialized laboratory techniques with mandatory attendance of 100% of the planned hours of the laboratories and direct learning through the use of instruments with the guidance of professionals belonging to the same Professional Profile. There are no individual study hours.

SYLLABUS/CONTENT

The program has been explained in the heading "EDUCATIONAL OBJECTIVES (DETAIL)"

TEACHERS AND EXAM BOARD

Exam Board

SONIA SCHIAVO (President)

CRISTINA CUSATO

ALESSANDRA SIGNORETTI (President and Coordinator of Integrated Course)

LESSONS

LESSONS START

The practical training course begins after the end of the 1st semester lessons from the month of January to the month of February and after the lessons of the second semester from the month of April-May to the month of September with a break in the month of August

Class schedule

The timetable for this course is available here: Portale EasyAcademy

EXAMS

EXAM DESCRIPTION

Verification of the ability to perform laboratory tests with description of the procedures for carrying out and the purposes of the test for clinical-diagnostic tests.

ASSESSMENT METHODS

The exam takes place at the end of the completion of the annual internship. The student must demonstrate that he has achieved the intended objectives.

Exam schedule