AIMS AND CONTENT

LEARNING OUTCOMES

Module “Industrial Microbiology”

The course aims to provide the concepts needed for a good knowledge of the microbial strains used in the fermentation processes.

Module “Fermentation Chemistry”

The course aims to provide the concepts needed for a good knowledge of fermentation processes. Particular attention is paid to the principles and technological aspects of industrial fermentations

TEACHING METHODS

Frontal lessonsand laboratory.

SYLLABUS/CONTENT

Module “Industrial Microbiology”

1) Microorganisms and production processes 2) Isolation of industrial microorganisms, production strains. 3) Materials for culture media, sources of carbon and nitrogen. 4) Development of the composition of industrial culture media, optimization techniques. 5) Mutants for industrial use and mutagenesis techniques 6) Long term storage  of cells , cell banking  of industrial strains. 7) Development of shake-flask  process , the role of oxygenation 8) Growth of microorganisms: bacteria, actinomycetes, yeasts and fungi,  biomass measurement techniques. 9) Analysis of Scale Up process: vegetative stage, prefermentation stage, the production stage. 10) Industrial techniques of the production phase: Batch, Fed-batch systems, flow control. 11) The equipment for fermentation, pH, pO2, Off gas and supervision systems (SCADA). 12) Downstream: recombinant proteins, antibiotics 13) Productions: Amino acids, organic acids, enzymes for industry 14) Recombinant Escherichia coli: solubility of recombinant proteins and inclusion  bodies 15) Streptomyces : Production of antitumor  and antibiotics 16) Yeasts: Saccharomyces cerevisiae, Pichia pastoris and Hansenula anomala . 17) Fungi : immunomodulators, antibiotics  and biomass production 18) Plant cells. production of alkaloids, production from algae: carotenoids, Omega3 fatty acids. 19) Biotransformation / Bioconversion: pharmaceutical intermediates (APIs) and fine chemicals 20) Energy from microbial biomass: bioethanol, biodiesel, biogas 21) Bioremediation of polluted  areas: hydrocarbons, chemicals. 22) biodepuration: microbiology of activated sludge.

Module “Fermentation Chemistry”

Part of “Fermentation Chemistry." 1. The fermentations: Commercial and metabolic classifications, Operation modes, Aeration, Agitation, Sterilization, Immobilization, 2. Fermentation plants: Mixed fermenter, Fixed bed bioreactor, Tower bioreactor, Waldhof fermenter, Volgelbusch fermenter, Rotating disk bioreactor, Immobilized-cell reactor, Bioreactor with dialysis membrane, Extractive bioreactor; 3. Recovery of fermentation products: Cell disintegration, Clariflocculation, Filtration, Sedimentation, Centrifugation, Membrane processes, Evaporation, Liquid-liquid extraction, Distillation, Adsorption, Precipitation, chromatography; 4. Kinetics of microbial growth: Batch and continuous cultures, Material balances and productivity of a CSTR; 5. Enzyme kinetics and thermodynamics; 6. Industrial fermentations: Glycolytic pathways, Krebs cycle, Respiration, Pentoses phosphates shunt, Homolactic and heterolactic Fermentations, Alcoholic fermentation, Acetic Fermentation, butyric and butylic alcohol fermentations, Mixed-acid fermentation, Propionic fermentations, Citric fermentation; 7. Production of antibiotics; 8. Fermentations of environmental interest; 9. Realization of a fermentation process in laboratory.

RECOMMENDED READING/BIBLIOGRAPHY

1. M. Marzona, “Chimica delle Fermentazioni & Microbiologia Industriale”, 2a edizione, Piccin Nuova Libraria S.p.A., Padova, 1996.
2. Matilde Manzoni, “Microbiologia Industriale”, Casa Editrice Ambrosiana, Milano, 2006.
3. Donadio Stefano e Marino Gennaro, “Biotecnologie Microbiche”, Casa Editrice Ambrosiana, Milano, 2008.
4. P. Barbieri, G. Bestetti, E. Galli e D. Zannoni, Microbiologia Ambientale ed Elementi di Ecologia Microbica, 1a edizione, Casa Editrice Ambrosiana, Milano, 2008

TEACHERS AND EXAM BOARD

Exam Board

ATTILIO CONVERTI (President)

GIANCARLO DONDO (President)

PATRIZIA PEREGO

LESSONS

LESSONS START

October 3, 2016.

Class schedule

INDUSTRIAL MICROBIOLOGY, BIOTECHNOLOGY OF FERMENTATIONS AND LABORATORY COURSE

EXAMS

EXAM DESCRIPTION

Oral.

ASSESSMENT METHODS

Module "Industrial Microbiology": Learning content from the students was found during the course, through questions and insights. At the end of the same it will be made an oral examination, both on topics discussed during the course, through the presentation of a paper in the form of theme. In it the student will analyze an industrial microbiology article selected on the basis of the arguments developed during the lessons.

Module "Fermentation" Chemistry: Learning content from the students was found either during the course, through questions and insights, that at the end of the same, through a) the presentation of a paper in the form of theme in which ' student elucidates 3 or 4 main topics of the course, b) the establishment of a review work on a topic chosen from those discussed in class, c) a final talk on topics discussed during the course.

Exam schedule