CODE 80808 ACADEMIC YEAR 2016/2017 CREDITS 4 cfu anno 3 BIOTECNOLOGIE 8756 (L-2) - SCIENTIFIC DISCIPLINARY SECTOR CHIM/01 TEACHING LOCATION SEMESTER 2° Semester TEACHING MATERIALS AULAWEB AIMS AND CONTENT LEARNING OUTCOMES The course provides an overview on sample pre-treatment procedures and on the main instrumental analytical techniques used in biotechnology. A particular attention is also devoted to the control of analytical data. TEACHING METHODS Frontal lessons SYLLABUS/CONTENT Sample pre-treatment: physical and chimical methods. Criteria for the choice of an analytical method; figures of merit. Chromatographic techniques: TLC, high-performance liquid chromatography (HPLC) and gas chromatography (GC). Evaluation parameters in chromatography. Optimisation of a chromatographic method. Block schemes of instrumental equipment. Introduction to spectroscopic methods. Ultraviolet-visible (UV-VIS) spectrophotometry; qualitative and quantitative applications. Principles of fluorescence spectroscopy. Cytofluorimetry. Principles of infrared (IR) spectroscopy. Mass spectrometry (MS): principles and applications. Components of a mass spectrometer. Ionisation modes. Analysers. Basic principles for interpretation of mass spectra. Nuclear magnetic resonance (NMR) spectroscopy. Principles of the technique. Information provided by a 1H-NMR spectrum. Quality control. Good laboratory practices (GLP). Validation of an analytical method. Errors. Certified reference materials. Significant figures. Control charts. RECOMMENDED READING/BIBLIOGRAPHY Skoog - Leary, Chimica Analitica Strumentale, Edises. Material provided in class and/or available on Aulaweb. TEACHERS AND EXAM BOARD PAOLO OLIVERI Ricevimento: In the Section of Pharmaceutical and Food Chemistry and Technologies of the Department of Pharmacy – DIFAR (Viale Cembrano, 4), upon appointment, to be agreed by e-mail (oliveri@difar.unige.it) o by phone (010 3532626). Exam Board PAOLO OLIVERI (President) MONICA CASALE RICCARDO LEARDI CRISTINA MALEGORI SILVIA SCHENONE LESSONS LESSONS START March 1, 2017. Class schedule INSTRUMENTATION AND CONTROL OF QUALITY EXAMS EXAM DESCRIPTION Oral. ASSESSMENT METHODS The course does not require midterm. The credits will be acquired by passing an oral examination. The oral examination is always conducted by two faculty members and has a duration of at least 30 minutes. With these modes, the commission is able to verify with high accuracy to achieve the educational objectives of teaching by putting different questions related to the program actually carried out during the course. When the educational objectives are not achieved, the student is invited to deepen his knowledge and to use any further explanation by the lecturer. Exam schedule Data appello Orario Luogo Degree type Note 22/06/2017 09:00 GENOVA Orale 06/07/2017 09:00 GENOVA Orale 21/07/2017 09:00 GENOVA Orale 15/09/2017 09:00 GENOVA Orale