CODE 70737 ACADEMIC YEAR 2021/2022 CREDITS 23 cfu anno 2 TECNICHE DI LABORATORIO BIOMEDICO 9293 (L/SNT3) - GENOVA SCIENTIFIC DISCIPLINARY SECTOR MED/46 LANGUAGE Italian TEACHING LOCATION GENOVA SEMESTER Annual MODULES Questo insegnamento è un modulo di: PRACTICAL-CLINICAL INTERNSHIP - 2ND YEAR TEACHING MATERIALS AULAWEB AIMS AND CONTENT AIMS AND LEARNING OUTCOMES Acquire knowledge and practical skills related to clinical chemistry, microbiology, immunometry, bacteriology, mycobacteriology, histology and cytology techniques. TEACHING METHODS Learning activities of specialized laboratory techniques with mandatory attendance of 100% of the planned hours of the laboratories and direct learning through the use of instruments with the guidance of professionals belonging to the same Professional Profile. There are no individual study hours. SYLLABUS/CONTENT Chemical-clinical sector 70 h: • Characteristics of serum, plasma, anticoagulants and practical exercise on the treatment of these biological materials • Features and operation of automated equipment for clinical chemistry determinations with practical training • Calibrations and Daily Quality Check (QC) • Ordinary and extraordinary equipment maintenance • Demonstration of the technical validation criteria of the values obtained, visualization of the quality control charts, of the methods and of the linearity of the reactions on the analyzers electrophoresis sector 70 h: • Execution of electrophoresis of serum proteins with Capillarys method in liquid phase. • Acquisition of the principle of equipment operation, preparation of reagents and quality controls • Visualization of serum, normal and pathological electrophoretic traces • Performing the zonal electrophoresis of urine on agarose gel • Preparation and use of buffer and dye for gel electrophoresis • Demonstration of serum and / or urinary immunofixation methods • Protein dosage with nephelometric method. Preparation of reagents, calibrations, quality controls and validation of results Immunometry sector 70 h: • Knowledge of the main immunometric researches • Acquisition of the main techniques applied in immunometry • Automation in immunometry, use of this in the daily routine • Importance of the accuracy of the data processed according to the control / calibration performed on the equipment in use • Detection and processing of data obtained from the analyzes, autonomous management of the results with consequent development of the control and / or confirmation methods • Interpretation of the data obtained • The error in immunometry: cause, treatment and remedy Microbiology sector 175 h: • Acquisition of bacteriology laboratory techniques: environments, gowns, disposable gloves, glasses and mask for special manipulations • Containers, collection, transport, acceptance and rejection of the sample sorting sample • Culture grounds: solid, liquid, enriched, selective, differential • Material sowing protocols • Interpretation of growths on the plates in relation to the seeded materials • Protocols for identification and antibiogram • Colony morphology and isolation on selective media • Preparation of slides and stains: Gram, Ziel Nilsen, Methylene blue, Indian ink • Manual and automatic identification, meaning of bionumero and serological identification indexes • Manual antibiogram and with the use of automatic systems, MIC, Etest • Anaerobic germs: culture in an oxygen-free atmosphere, identification • Mycology yeasts and aerial mycelial forms recognition • Protection and safety in cultures for mycobacteria • Sample decontamination and sowing in solid and liquid soils • Gene amplification techniques Pathological anatomy sector 175 h: • Registration and filing of the material • Storage and fixation of the material • Sampling • Paraffin embedding techniques • Cutting techniques • Cytological preparations for exfoliative cytology • Cytological preparations of liquids • Basic histological staining TEACHERS AND EXAM BOARD ALESSANDRA SIGNORETTI Ricevimento: On appointment DISC pathological university anatomy second floor traineeship coordinator's office a.signoretti@edu.unige.it +390103537807 Exam Board CRISTINA CUSATO (President) ALESSANDRA SIGNORETTI (President) NICOLA TRAVERSO (President) LESSONS LESSONS START The practical training course begins after the end of the 1st semester lessons from the month of January to the month of February and after the lessons of the second semester from the month of April-May to the month of September with a break in the month of August Class schedule The timetable for this course is available here: Portale EasyAcademy EXAMS EXAM DESCRIPTION Verification of the ability to perform laboratory tests with description of the procedures for carrying out and the purposes of the test for clinical-diagnostic tests. ASSESSMENT METHODS The exam takes place at the end of the completion of the annual internship. The student must demonstrate that he has achieved the intended objectives. Exam schedule Data appello Orario Luogo Degree type Note 25/02/2022 14:30 GENOVA Laboratorio