CODE 75096 ACADEMIC YEAR 2022/2023 CREDITS 1 cfu anno 1 TECNICHE DI LABORATORIO BIOMEDICO 9293 (L/SNT3) - GENOVA SCIENTIFIC DISCIPLINARY SECTOR BIO/10 LANGUAGE Italian TEACHING LOCATION GENOVA SEMESTER 2° Semester MODULES Questo insegnamento è un modulo di: PHYSIOLOGY AND BIOCHEMISTRY TEACHING MATERIALS AULAWEB OVERVIEW The course consist of two modules: Propedeutical Biochemistry and Biochemistry, and is divided into two parts: a part of lessons in presence and one part, as support of the first, consisting of online classes. AIMS AND CONTENT LEARNING OUTCOMES The first part of the course aims to provide the basis of general and organic chemistry to address Biochemistry, the second part of the course. The aim of this second part is both the acquisition of knowledge about the structure and function of proteins and enzymes proteins as the main pathways of carbohydrate, fat, and protein catabolism. AIMS AND LEARNING OUTCOMES The first part of the course aims to provide the basis of general and organic chemistry to address Biochemistry, the second part of the course. The aim of this second part is both the acquisition of knowledge about the structure and function of proteins and enzymes proteins as the main pathways of carbohydrate, fat, and protein catabolism. TEACHING METHODS Lectures in presence,supported / supplemented by recorded lectures that the students can access online through the platform AulaWeb SYLLABUS/CONTENT PROPEDEUTICAL BIOCHEMISTRY solutions. Properties and units of measure: molarity, normality,% w / w% w / v. osmotic pressure. The chemical reactions. chemical balance. chemical kinetics. Reaction speed. Catalysis and enzymatic catalysis, Km. Spontaneity of reactions, free energy. Ionization of water. pH. Acidity and alkalinity. Strong and weak acids and bases, hydrolysis, buffers. The chemistry of carbon, the carbon valence. The functional groups. Alcohols and thiols. Redox reactions. Oxidation of alcohols and thiols. Aldehydes and ketones. Hemiacetals. Amines. Carboxylic acid. Amides, esters and anhydrides. Fatty acids. Triglycerides. Phospholipids. Biomolecules and their functional groups. Carbohydrates: Monosaccharides: glucose, fructose, ribose and deoxyribose, disaccharides: sucrose, and polysaccharides, cellulose, glycogen. Amino acids. Peptides and proteins. The peptide bond. Biochemistry: Protein Structure, Myoglobin. Hemoglobin. Enzymes: structure and function. Catabolism and anabolism: general concepts of the intermediate metabolism. Glycolysis, gluconeogenesis and glucose: regulation. Lactate cycle. Krebs cycle. Strategies for ATP synthesis. Synthesis and degradation of glycogen. Oxidation of fatty acids. Chetonic bodies. Amino acids catabolism: urea cycle. RECOMMENDED READING/BIBLIOGRAPHY Catani, Savini, Guerrieri, Avigliano “Appunti di Biochimica per le lauree triennali” Editore: Piccin Stefani, Taddei “Chimica biochimica e biologia applicata” Editore: Zanichelli TEACHERS AND EXAM BOARD MARIO PASSALACQUA Ricevimento: Dr. Mario Passalacqua, Viale Benedetto Xv,1 16132 Genova Tel 010-3538128 e-mail: Mario.Passalacqua@unige.it Exam Board MONICA AVERNA (President) PIETRO BALDELLI (President) PIERLUIGI VALENTE MARIO PASSALACQUA (President and Coordinator of Integrated Course) LESSONS LESSONS START II SEMESTER – STARTING FROM MARCH 2017 Class schedule The timetable for this course is available here: Portale EasyAcademy EXAMS EXAM DESCRIPTION Oral examination ASSESSMENT METHODS For the units with video lessons, self-assessment tests, which are designed to stimulate students in learning, are provided. Self-assessment tests are useful also to verify the correct understanding, facilitating the preparation of the final exam, done in the presence.
