CODE | 68478 |
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ACADEMIC YEAR | 2022/2023 |
CREDITS |
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SCIENTIFIC DISCIPLINARY SECTOR | MED/46 |
LANGUAGE | Italian |
TEACHING LOCATION |
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SEMESTER | 1° Semester |
MODULES | This unit is a module of: |
TEACHING MATERIALS | AULAWEB |
Clinical Chemistry
Electrophoresis
• Introduction to the Electrophoresis and Specific Protein Sector: biological materials and collection; anticoagulants; treatment, conservation and centrifugation; identification of biological samples.
• Electrophoretic session setup: electrophoresis principle and methods; Equipment and accessories (power supply, electrophoretic chamber, buffer, dye, densitometer); Stages of electrophoretic running (application, migration, coloring, detection).
• Electrophoretic separation of proteins: standard electrophoretic profile; Fractions (albumin-α1-α2-β1-β2-γ): Specific proteins found in the electrophoretic tracing.
• IFE (gel) electrophoretic immunofixation
• Bence Jones Protein Research (gel)
• Automatic electrophoresis analyzers
Online or on-site lessons, depending on covid guidelines, with slides available on Aula Web
Clinical Chemistry 1.Introduction to the Laboratory: definition of the pre-analytical phase, blood draw and not acceptability criterion of the sample; type of samples; anticoagulants; treatment, transportation, centrifugation and storage of samples. 2. Instrumental analytical techniques: photometric characteristics of light, interaction between matter and radiant energy, Lambert – Beer law, absorbance and calibration curve, zeroing against blank; general principles of photometry and spectrophotometry; single beam spectrophotometer, double beam spectrophotometer; turbidimetry; nephelometry. 3. Clinical applications: spectrophotometry with direct methods, spectrophotometry with indirect methods; colorimetric reactions; enzymatic reactions, primary reactions and couple reactions, NAD and NADP dependent enzymatic reactions; enzymatic- colorimetric reactions, Trinder end point method. 4 Indirect potentiometry: principle of this method. 5. Methods of determination of important tests about clinical relevance (SGOT, SGPT, gGT, PCE and dibucaine number, LDH, Total and Direct Bilirubin, CPK, Alkaline Phosphatase, Amylase and Pancreatic Amylase, Lipase, Urea and BUN, Creatinine, Uric Acid, calculation of clearance endogenous, Iron, Ferritin, Glucose, total Cholesterol, HDL-C and LDL-C, Triglyceride, APO A B, Sodium, Potassium, Chloride, Calcium, phosphorous, Magnesium): Methods, interpretation of clinical data and interferences. 6. Quality assessment: precision and accuracy of the analytical date; specificity and sensitivity of the method; types of error; calibration: definition of detection limit and levels detectable; internal quality assessment, external quality assessment; dilutions. Electrophoresis
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Office hours: Dipartimento Ricerca Traslazionale, Medicina di Laboratorio, Diagnostica e Servizi U.O.C. Laboratorio Analisi IRCCS Istituto Giannina Gaslini Via Gerolamo Gaslini 5, 16147 Genova Tel.: 01056362823 Email: lauramarcomini@gaslini.org
Office hours: U.O.C Laboratorio di Analisi IRCCS Istituto Giannina Gaslini5, 16147 Genova Tel: 01056362834 E-mail elisabettafraternale@gaslini.org
MARIO PASSALACQUA (President)
ELISABETTA FRATERNALE
LAURA MARCOMINI
MADDALENA MASTROGIACOMO
ELENA ZOCCHI (President and Coordinator of Integrated Course)
For lessons start and timetable go to the link: https://easyacademy.unige.it/portalestudenti/
Please check the module Aulaweb page for timetable updates dependent on the sanitary and epidemic situation.
Multiple choice exam of the knowledge acquired, among which the definition of the basic elements of the subject.
Test in classroom by multiple choice assessment questionnaire.
Date | Time | Location | Type | Notes |
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11/01/2023 | 14:00 | GENOVA | Scritto | |
01/02/2023 | 14:00 | GENOVA | Scritto | |
17/02/2023 | 14:00 | GENOVA | Scritto | |
09/06/2023 | 14:00 | GENOVA | Scritto | |
05/07/2023 | 14:00 | GENOVA | Scritto | |
19/07/2023 | 14:00 | GENOVA | Scritto | |
15/09/2023 | 14:00 | GENOVA | Scritto |