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ANALYTICAL INSTRUMENTATION AND CONTROL OF QUALITY

CODE 80808
ACADEMIC YEAR 2022/2023
CREDITS
  • 4 cfu during the 3nd year of 8756 BIOTECNOLOGIE (L-2) - GENOVA
    		•
    SCIENTIFIC DISCIPLINARY SECTOR CHIM/01
    LANGUAGE Italian
    TEACHING LOCATION
  • GENOVA
    		•
    SEMESTER 2° Semester
    TEACHING MATERIALS AULAWEB

    AIMS AND CONTENT

    LEARNING OUTCOMES

    The course provides an overview of the sampling procedures and instrumental analytical techniques used in biotechnology. Several aspects of the quality control will be treated also.

    TEACHING METHODS

    Frontal lessons

    SYLLABUS/CONTENT

    1. Sample pre-treatment: physical and chimical methods.
    2. Criteria for the choice of an analytical method; figures of merit.
    3. Chromatographic techniques: TLC, high-performance liquid chromatography (HPLC) and gas chromatography (GC). Evaluation parameters in chromatography. Optimisation of a chromatographic method. Block schemes of instrumental equipment.
    4. Introduction to spectroscopic methods. Ultraviolet-visible (UV-VIS) spectrophotometry; qualitative and quantitative applications. Principles of fluorescence spectroscopy. Cytofluorimetry. Principles of infrared (IR) spectroscopy.
    5. Mass spectrometry (MS): principles and applications. Components of a mass spectrometer. Ionisation modes. Analysers. Basic principles for interpretation of mass spectra.
    6. Nuclear magnetic resonance (NMR) spectroscopy. Principles of the technique. Information provided by a 1H-NMR spectrum.
    7. Quality control. Good laboratory practices (GLP). Validation of an analytical method. Errors. Certified reference materials. Significant figures. Control charts.

    RECOMMENDED READING/BIBLIOGRAPHY

    Skoog - Leary, Chimica Analitica Strumentale, Edises.
    Material provided in class and/or available on Aulaweb.

    TEACHERS AND EXAM BOARD

    Exam Board

    PAOLO OLIVERI (President)

    MONICA CASALE

    RICCARDO LEARDI

    CRISTINA MALEGORI

    LESSONS

    Class schedule

    ANALYTICAL INSTRUMENTATION AND CONTROL OF QUALITY

    EXAMS

    EXAM DESCRIPTION

    Oral.

    ASSESSMENT METHODS

    The course does not require midterm. The credits will be acquired by passing an oral examination. The oral examination is always conducted by two faculty members and has a duration of at least 30 minutes. With these modes, the commission is able to verify with high accuracy to achieve the educational objectives of teaching by putting different questions related to the program actually carried out during the course. When the educational objectives are not achieved, the student is invited to deepen his knowledge and to use any further explanation by the lecturer.

    Exam schedule

    Date Time Location Type Notes
    18/01/2023 09:30 GENOVA Orale
    08/02/2023 09:30 GENOVA Orale
    16/06/2023 09:30 GENOVA Orale
    07/07/2023 09:30 GENOVA Orale
    21/07/2023 09:30 GENOVA Orale
    01/09/2023 09:30 GENOVA Orale
    15/09/2023 09:30 GENOVA Orale