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CODE 106999
ACADEMIC YEAR 2023/2024
CREDITS
SCIENTIFIC DISCIPLINARY SECTOR BIO/17
TEACHING LOCATION
  • GENOVA
SEMESTER 2° Semester
TEACHING MATERIALS AULAWEB

OVERVIEW

Histology studies solid and liquid tissues. Due to their different characteristics, these tissues require different methodologies to be investigated that must be selected also taking into account the aim of the study for which they are used.

The various methodologies applicable to different healthy and pathological tissues can be used in many fields of biology and medicine including basic and applied research.

AIMS AND CONTENT

LEARNING OUTCOMES

The course aims to provide basic training for a suitable selection of histological samples, the choice of the most useful methodologies for the analysis of the different samples and the correct interpretation of the data obtained. The learning outcomes  also include the acquisition of skills for: i) the isolation and preparation of histological samples that can be analyzed by microscopy or flow cytometry ii) the acquisition, data analysis and evaluation of their biological significance.

AIMS AND LEARNING OUTCOMES

Attendance and participation in learning outcomes  (class teaching) will allow the student to acquire the necessary knowledge to learn the methodological path aimed at the analysis of cells isolated from healthy and pathological histological samples, in particular tumor samples.   Specifically, at the end of the lessons the student:

1) will know the different possible methodological approaches for sample collection (solid and liquid sample)

2) will be able  to evaluate the suitability of the sample taking into account its histological characteristics 

3) will have acquired the main skills related to the methodological approaches for  ex-vivo samples processing derived from solid or liquid tissues 

4) will know the techniques of particular cell types isolation (example: lymphocytes infiltrating tumor tissues and /or tumor cells themselves) from ex vivo samples derived from solid or liquid tissues and will be able to evaluate the effectiveness of the various isolation methods.

5) will be able to apply the different methodologies useful for the phenotypic and functional study of cells derived from ex-vivo samples, derived from solid or liquid tissues and will be able to analyze the merits and limits of the different applicable methodologies (microscopic and flow cytometric techniques) 

6) will know other in vitro tissue study models (e.g. 3D models). 

7) will be able to analyze and interpret the data obtained from the application of the methodologies illustrated to the different types of tissue samples. 

During the course lessons, students will be offered some assessment tests of the acquired knowledge to which students will respond individually and anonymously. The topics emerging from the test will be discussed together with the teacher.

PREREQUISITES

To effectively address the teaching contents basic knowledge of cytology and histology are required.

TEACHING METHODS

The course includes 2 CFU (16 hours) of class teaching.

It is possible that the method of conducting the lessons is changed due to emergency situations that prevent the performance of the lessons in attendance. In this case the lessons will be carried out through the Teams platform.

 

SYLLABUS/CONTENT

Methods for the study of healthy and pathological tissues in basic and applied research:

- collection and processing of healthy tissue samples and samples derived from solid and haematological tumors (e.g. neuroblastoma, colon carcinoma, leukemia)

- techniques for the isolation of tumor tissue cells

- separation of specific cell populations from the leukocyte infiltrate

- insights into techniques for studying the phenotype and functions of cells derived from tumor samples

- microscopic and flow cytometric analyses for the observation of histological samples

- collection, validation and interpretation of the data obtained

RECOMMENDED READING/BIBLIOGRAPHY

The slides used during the lessons will be made available in PDF format together with other bibliographic material.

TEACHERS AND EXAM BOARD

Exam Board

ALESSANDRA DONDERO (President)

MARIELLA DELLA CHIESA

LESSONS

LESSONS START

(March 2024).

For lessons start and timetable go to the link: https://easyacademy.unige.it/portalestudenti/

Please check the module Aulaweb page for timetable updates dependent on the sanitary and epidemic situation.

EXAMS

EXAM DESCRIPTION

Oral examination

The oral exam is aimed at evaluating the learning of knowledge related to the topics illustrated and discussed in the classroom and the ability to integrate and contextualize this knowledge.

In case of certification of specific learning disability, disability or other special educational needs, the University legislation and regulations on disability and SLD are applied (https://unige.it/disabilita-dsa/studenti-disabilit%C3% A0-normative).

See also the "Other information" section of this syllabus.

It is possible that emergency situations prevent the students to taking part in the examination in attendance for the final evaluation. In this case, the Teams platform will be used. (https://cedia.unige.it/sites/cedia.unige.it/files/pagine/TEAMS%20mini%20guida%20IT%20Studente.pdf)

ASSESSMENT METHODS

The oral examination will mainly focused on the topics covered during the class teaching and will aim to verify the acquisition of knowledge and skills included in the learning outcomes

In addition, a written paper carried out by students independently on the teacher's track will be discussed, aimed at verifying the ability to analyze and interpret experimental data relating to the study of tissue samples.

It is possible that emergency situations prevent the students to taking part in the examination in attendance for the final evaluation. In this case, the examination procedures will be the same but the Teams platform will be used.

FURTHER INFORMATION

In case of certification of specific learning disability, disability or other special educational needs, please contact both the Department contact person, Prof. Sara Ferrando, and the lecturer at the beginning of the lectures to agree on teaching and exam methods which, in compliance of the teaching objectives, take into account individual learning methods and provide suitable compensatory/dispensatory tools recognized by the University SLD Student Service.