The part relating to the Medical Technical Sciences, in line with the disciplines of the topics covered in the Clinical Pathology part, is entrusted to Professors belonging to the professional profile of Biomedical Laboratory Technician, focusing attention on analytical techniques.
At the end of the integrated course, the student faces and acquires concepts that allow him to know the emergency laboratory, its clinical importance, the timeliness of the results, the tests that are performed, the dosage methods, the tests with forensic value and the legal aspects of these tests. He also acquires knowledge on methods of dosing specific tests for myocardial infarction. Acquires skills on the execution of I and II level tests relating to coagulation, on the dosage of immunosuppressant drugs and on the execution of blood gas analysis and its clinical significance. Knowledge of the different specialized branches of the laboratory. acquisition and knowledge of the organization of a laboratory and of the phases that make up the process of analyzing a sample; the pre-analytical, analytical and post-analytic phase; errors in the laboratory; internal quality controls and EQAs. Techniques in clinical chemistry: turbidimetry spectrophotometry, nephelometry, enzymatic and potentiometric reactions. The main methods used for dosages in clinical chemistry and their clinical significance; urine test; hints of health legislation.
Acquisition of notions that allow to know the hematology laboratory and its clinical importance in the diagnostic process. The old dosing methods for carrying out the blood count compared with the new automated methods, which are faster, more accurate and more sensitive which allow, by reading the flags and cytograms, to verify the instrumental results. Identification of haematological pathologies and execution of the peripheral blood smear; acquisition of knowledge on some analytical techniques used for the diagnosis of pathological hemoglobins, other diagnostic techniques related to the screening of 1st level hemoglobinopathies, analytical techniques related to the diagnosis of other biological fluids analyzed in the hematology sector. During the process of analyzing the blood count and other hematological tests, the student acquires notions on the use of quality controls dedicated to the sector.
Classroom lessons
Medical Technical Sciences Hematology Techniques (1 CFU) • In vitro diagnostics: valuable information for clinical diagnosis Quality control in hematological in vitro diagnostics, • Elements of blood physiopathology • Hematological diagnostic techniques • Blood smear and standard staining • Reactive changes in blood cells • Hemoglobin Assets, Hemoglobinopathies diagnostic techniques • CSF, cavity effusions and synovial fluid. Diagnostic techniques for cell counting • E.V.S. , Diagnostic techniques • Fecal occult blood screening • Lymphocyte typing Medical Technical Sciences Dr.sa Cristina Cusato (1 CFU) • The emergency laboratory: description of an emergency laboratory. Tests performed in the emergency laboratory and their importance. The Principles of Methods. The Tat (Response Time) . The organization of work. • Coagulation: Description of methods of analysis. The coagulometer, the normal values of the coagulation tests. • Chain of custody: Legal aspects and forensic value of tests. Principles of blood alcohol assay methods and toxicological tests. Nonconformity and its importance. • Immunosuppressive drugs: description of tests and principles of dosing methods. Clinical Importance. Differences in use among the various immunosuppressants. • Blood gas analysis: description of tests and principles of dosage methods; principles of acid-base balance. Poct (point of care). • Cardiac enzymes: troponins and Bnp. Differences between new and old assay methods and tests. Normal values and their clinical significance.
Medical Technical Sciences Clinical Chemistry Techniques (1 CFU) • Instrumental analytical techniques: double beam spectrophotometry, nephelometry, turbidimetry, enzymatic reactions, indirect potentiometry and Trinder method. • Main dosages in Clinical Chemistry: AST, ALT, gGT, PCE and dibucaine number, LDH, total and direct Bilirubin, CPK, ALP, Total and Pancreatic Amylase, Lipase, Urea and Ureic Nitrogen, Creatinine, Uric Acid, clearance calculation endogenous, Iron, Ferritin, Glucose, Total cholesterol, HDL and LDL, Triglycerides, APO A and B, Sodium, Potassium, Chlorine, Calcium, Phosphorus, Magnesium. • Interpretation of clinical data and interferences. • Serum indices and relative interferences • Insights on the quality of the analytical system (the phases of the pre-analytical, analytical and post-analytical process, specificity, sensitivity, accuracy and precision) • The pre-analytical variables that can compromise the reliability of the result. Medical Technical Sciences Dr.sa Alessandra Signoretti (1 CFU): • Introduction to Analysis Laboratories. • Anticoagulants and sampling methods • The phases of the pre-analytical, analytical and post-analytical process (specificity, sensitivity, accuracy and precision). • Laboratory errors, quality controls, calibration. • Light and its characteristics. • Spectrophotometry • Dilutions. • Physico-chemical examination of the urine • Hints of health legislation
Biochimica clinica e medicina di laboratorio, Autori: Marcello Ciacci, Giuseppe Lippi
ISBN 8879599585, 9788879599580- Edises, 2017 (acquisto obbligatorio).
Lecture notes
Ricevimento: By appointment Internship Coordinator Dr.sa Alessandra Signoretti alessandra.signoretti@edu.unige.it Tel 0103357807 By appointment Tutor Silvia Naso silvia.nasi@hsanmartino.it By appointment Tutor Cristina Cusato cristina.cusato@hsanmartino.it
Ricevimento: Dipartimento Ricerca Traslazionale, Medicina di Laboratorio, Diagnostica e Servizi U.O.C. Laboratorio Analisi IRCCS Istituto Giannina Gaslini Via Gerolamo Gaslini 5, 16147 Genova Tel.: 01056362823 Email: lauramarcomini@gaslini.org
CRISTINA CUSATO (President)
NICOLA TRAVERSO (President)
LAURA MARCOMINI
VALENTINA PORCILE
MARGARITA RUMBULLAKU
VALERIA VISCONTI
ALESSANDRA SIGNORETTI (President and Coordinator of Integrated Course)
I year II semester
Verification with assessment methods in oral form
Students must demonstrate that they have acquired the skills related to all MED / 46 courses.
If the candidate has not achieved the minimum objectives, the exam will not be deemed passed
