AIMS AND CONTENT

AIMS AND LEARNING OUTCOMES

The internship of the third year of the course provides for 560 hours of student activity in specialist laboratories of hospitals or local health authorities affiliated throughout the region with the University of Genoa in order to acquire skills with the purely practical activity envisaged by the profile of the technician of Biomedical Laboratory and the Manifesto of the studies of the course. The internship program is divided into specialist sectors in periods which provide for attendance of between two and five weeks of stay. Before the professional internship, students must acquire 1 preparatory laboratory credits with compulsory attendance.

Acquire knowledge and practical skills related to molecular biology techniques, immunohaematological, immunohistochemical, chromatographic and flow cytometric techniques.

TEACHING METHODS

Learning activities of basic laboratory techniques with compulsory attendance of 100% of the planned hours of the laboratories and direct learning of laboratory techniques through the use of instruments with the guidance of professionals belonging to the same professional profile. There are no individual study hours.

SYLLABUS/CONTENT

Pathological anatomy sector 140 h:

• Immunohistochemical techniques

• Preparatory treatment of the material for immuno-histochemical examinations

• Basic immunohistochemical techniques (manual, semi-automatic and automatic)

• Main markers in immuno-histochemistry (data-sheet reading, optimal antibody new dilution, etc.)

• Use of positive and negative controls

• Preparation of tampons; tool maintenance and management

• Immuno-histochemistry on cytological material

• Preparation of preparations for extemporaneous diagnostics - Elective stains for intraoperative examinations - cytological preparation for intraoperative examination

• In situ hybridization techniques (FISH –CISH-SISH); manual, semi-automatic, and automatic techniques

• Preparatory treatment of the material for FISH analysis (histological, cytological, decolored, peripheral blood, bone marrow aspirate)

• Main types of probes reading the data sheet and setting up the methods for new reagents

• Use of controls (probe with internal control); tool maintenance and management.

• Microscopic evaluation of the preparations carried out and their adequacy, any changes to be made

Molecular biology sector 175 h:

• Organization of areas and behavioral norms in the molecular biology laboratory

• Nucleic acid extraction with manual and automatic methods used in the laboratory

• Dosage and evaluation of nucleic acid quality

• PCR techniques, GEL electrophoresis

• Sequencing

• Interpretation of results

• Maintenance and use of laboratory instruments

• CQI and VEQ quality controls

Immunohematology sector 140 h:

• Blood group on adults; Blood group + TCD on newborns

• Execution of phenotype Rh

• Weak “D” research test execution

• Direct / fractionated Coombs test; Indirect Coombs test

• Research and identification of anti-erythrocyte antibodies

• Elution of autoantibodies

• Automation in immunohaematology;

• Role and activities of a TSLB in a SIT

• Emovigilance and security

• Adverse reactions

CYTOFLUORIMETRY OR CHROMATOGRAPHY SECTOR 105 H:

Flow cytometry

• Knowledge of the physical and technological bases of flow cytometry: general scheme of a flow cytometer (fluidics, optics, electronics); significance of the cellular physical parameters evaluated in cytometry; fluorescence signals; description of fluorochromes; clearing operation; meaning of multiparametric analysis; gating strategy; data representation

• Direct and indirect coloring techniques of biological samples

• Main application methodologies for the study of phenotype and lymphocyte functions, such as cytokine production and proliferation

Chromatography

• Demonstration and characteristics of the functioning of the modules of an HPLC

• Extraction of a sample on a resin column

• Use of the S.I. in different methods

• Reading of a chromatogram and possible changes to the retention times of the peaks

• Intervention on the reading program to highlight and recognize the various operations in order to recognize a substance in the analytical phase

• Exercise on the various methods in the elution phase of a sample

• Procedure for stabilizing a column and identifying the various columns on an HPLC module

• Demonstration and operation of an LC-MS / MS; extraction of a sample for analysis in LC-MS / MS

• Evaluation of an analysis calibration curve in LC-MS / MS

TEACHERS AND EXAM BOARD

Exam Board

CRISTINA CUSATO (President)

SILVIA NASO (President)

NICOLA TRAVERSO (President)

ALESSANDRA SIGNORETTI (President and Coordinator of Integrated Course)

LESSONS

LESSONS START

The practical internship begins after the end of the lessons of the first semester from December to February and after the lessons of the second semester from April-May to September with a break in August.

Class schedule

The timetable for this course is available here: Portale EasyAcademy

EXAMS

EXAM DESCRIPTION

At the end of the curricular internship path with a positive result, the student will have to take the annual internship exam in September with the execution of laboratory tests.

The exam will take place in the teaching laboratory on the skills acquired during the preparatory laboratory and the curricular internship.

ASSESSMENT METHODS

Verification of the ability to carry out laboratory tests with a description of the methods of carrying out and of the purposes of the test for clinical-diagnostic investigations.

FURTHER INFORMATION

Students can request information and support from the Internship Coordinator and Clinical Tutors