The course is aimed at presenting the main biomolecular typing techniques currently used in Pathological Anatomy mainly but not limited to Precision Medicine and individualized therapies.
At the end of the course the student will acquire knowledge on the main molecular markers of diagnosis, prognosis and therapeutic indication currently used in a Precision Medicine context. The student will also acquire skills on the main investigation methods for the aforementioned markers and on the organization of workflows in a molecular pathology laboratory
The student will acquire expertise in the management of the molecular pathology laboratory connected to pathological anatomy, in the pre-analytical, analytical and post-analytical phases including quality controls on the main investigation systems and on the biological and clinical characteristics of the main markers
Preliminary knowledge of molecular biology and pathological anatomy
Workflows in Pathological Anatomy
Precision Medicine
General information on ancillary methods in Pathological Anatomy
The Mutations
Structural organization of a molecular pathology laboratory
The Microdissection
The extraction of nucleic acids
Storage of nucleic acids
Southern Blots
Northern Blots
Western blot
Other blotting methods
PCR and Variants
Classic sequencing
Next Generation Sequencing
The main molecular markers (ALK, BRAF, BRCA, EGFR, HER2/neu, TRAK, RAS et al)
ROBERTO FRANCO FIOCCA (President)
FEDERICA GRILLO (President)
LUCA MASTRACCI (President)
GIORGIA ANSELMI
MARIANGELA LANATA
VALERIO GAETANO VELLONE (President and Coordinator of Integrated Course)
MOLECULAR BIOLOGIC TECHNIQUES IN PATHOLOGIC ANATOMY
