OVERVIEW

The course is aimed at presenting the main biomolecular typing techniques currently used in Pathological Anatomy mainly but not limited to Precision Medicine and individualized therapies.

AIMS AND CONTENT

LEARNING OUTCOMES

At the end of the course the student will acquire knowledge on the main molecular markers of diagnosis, prognosis and therapeutic indication currently used in a Precision Medicine context. The student will also acquire skills on the main investigation methods for the aforementioned markers and on the organization of workflows in a molecular pathology laboratory

AIMS AND LEARNING OUTCOMES

The student will acquire expertise in the management of the molecular pathology laboratory connected to pathological anatomy, in the pre-analytical, analytical and post-analytical phases including quality controls on the main investigation systems and on the biological and clinical characteristics of the main markers

PREREQUISITES

Preliminary knowledge of molecular biology and pathological anatomy

SYLLABUS/CONTENT

Workflows in Pathological Anatomy

Precision Medicine

General information on ancillary methods in Pathological Anatomy

The Mutations

Structural organization of a molecular pathology laboratory

The Microdissection

The extraction of nucleic acids

Storage of nucleic acids

Southern Blots

Northern Blots

Western blot

Other blotting methods

PCR and Variants

Classic sequencing

Next Generation Sequencing

The main molecular markers (ALK, BRAF, BRCA, EGFR, HER2/neu, TRAK, RAS et al)

TEACHERS AND EXAM BOARD

Exam Board

ROBERTO FRANCO FIOCCA (President)

FEDERICA GRILLO (President)

LUCA MASTRACCI (President)

GIORGIA ANSELMI

MARIANGELA LANATA

VALERIO GAETANO VELLONE (President and Coordinator of Integrated Course)

LESSONS

Class schedule

MOLECULAR BIOLOGIC TECHNIQUES IN PATHOLOGIC ANATOMY

EXAMS

Exam schedule