CODE 73161 ACADEMIC YEAR 2023/2024 CREDITS 2 cfu anno 3 TECNICHE DI LABORATORIO BIOMEDICO 9293 (L/SNT3) - GENOVA SCIENTIFIC DISCIPLINARY SECTOR MED/46 LANGUAGE Italian TEACHING LOCATION GENOVA SEMESTER 1° Semester MODULES Questo insegnamento è un modulo di: APPLIED MEDICAL SCIENCE AND CHROMATOGRAPHIC TECHNIQUES TEACHING MATERIALS AULAWEB AIMS AND CONTENT AIMS AND LEARNING OUTCOMES The Chromatography module proposes the study of the fundamental principles of the technique and of the detection systems. The attention is focused on Mass Spectrometry, Gas Chromatography, techniques widely used in work activities. The basic principles provided will facilitate the internships by introducing the student to the working world. In particular: Provide the theoretical foundations related to Chromatography and Chromatographic Techniques and Detection Systems To acquire the fundamental principles of Mass Spectrometry, Gas Chromatography and Gas Chromatography-Mass Spectrometry, Liquid Chromatography-Mass Spectrometry To make known the lines of the Validation Procedure of a chromatographic method Know the main applications of chromatographic methods in the biomedical laboratory Provide the ability to perform HPLC chromatographic analysis methods Therefore the student will have to acquire the basic concepts of the functioning of chromatographic techniques in order to be able to autonomously evaluate how to apply them to assist in the clinical diagnosis of specific pathologies TEACHING METHODS Frontal lessons in the classroom with projected slide presentations and simulation of practical applications. SYLLABUS/CONTENT Introduction to Chromatography Matter and substances States of aggregation of matter and changes of state Classification of chromatographic techniques Gas chromatography and detectors HPLC and detectors Gas chromatography-mass spectrometry Liquid chromatography-mass spectrometry Quantitative methods in chromatography Validation of a chromatographic method Applications of chromatographic methods in the biomedical laboratory RECOMMENDED READING/BIBLIOGRAPHY We propose the study of what is reported in the slides presented and discussed. TEACHERS AND EXAM BOARD LAURA BARBAGALLO MADDALENA MASTROGIACOMO Exam Board DANIELE REVERBERI (President) LAURA BARBAGALLO NIDIA SOFIA DIAZ GAITAN MADDALENA MASTROGIACOMO (President and Coordinator of Integrated Course) LESSONS Class schedule CHROMATOGRAPHIC TECHNIQUES EXAMS EXAM DESCRIPTION The exam can take place in oral form through discussion of all the topics covered or in written form as a multiple choice test ASSESSMENT METHODS The evaluation of the student will focus on the assessment of the knowledge transmitted through the lessons and on the ability to link the application of the techniques to the diagnosis of pathologies Exam schedule Data appello Orario Luogo Degree type Note 26/01/2024 14:00 GENOVA Scritto + Orale l'esame sarà alle ore 14:00 nell'atleta di patologia generale 19/02/2024 14:00 GENOVA Scritto + Orale appello di febbraio in aula B del Polo Alberti 17/06/2024 14:00 GENOVA Scritto + Orale aula ex patologia (edificio 7) 08/07/2024 14:00 GENOVA Scritto + Orale studio Prof. Mastrogiacomo lab di bioterapie CBA 2°piano torre C 05/09/2024 14:00 GENOVA Scritto + Orale Agenda 2030 - Sustainable Development Goals Good health and well being