CODE 75095 ACADEMIC YEAR 2023/2024 CREDITS 1 cfu anno 1 TECNICHE DI LABORATORIO BIOMEDICO 9293 (L/SNT3) - GENOVA SCIENTIFIC DISCIPLINARY SECTOR BIO/10 LANGUAGE Italian TEACHING LOCATION GENOVA SEMESTER 2° Semester MODULES Questo insegnamento è un modulo di: PHYSIOLOGY AND BIOCHEMISTRY TEACHING MATERIALS AULAWEB OVERVIEW The course consist of two modules: Propedeutical Biochemistry and Biochemistry, and is divided into two parts: a part of lessons in presence and one part, as support of the first, consisting of online classes. AIMS AND CONTENT LEARNING OUTCOMES The first part of the course aims to provide the basis of general and organic chemistry to address Biochemistry, the second part of the course. The aim of this second part is both the acquisition of knowledge about the structure and function of proteins and enzymes proteins as the main pathways of carbohydrate, fat, and protein catabolism. AIMS AND LEARNING OUTCOMES The first part of the course aims to provide the basis of general and organic chemistry to address Biochemistry, the second part of the course. The aim of this second part is both the acquisition of knowledge about the structure and function of proteins and enzymes proteins as the main pathways of carbohydrate, fat, and protein catabolism. TEACHING METHODS The lessons will be carried out in presence. Please check the module Aulaweb page for timetable updates dependent on the sanitary and epidemic situation. SYLLABUS/CONTENT PROPEDEUTICAL BIOCHEMISTRY solutions. Properties and units of measure: molarity, normality,% w / w% w / v. osmotic pressure. The chemical reactions. chemical balance. chemical kinetics. Reaction speed. Catalysis and enzymatic catalysis, Km. Thermodynamics. Concepts of enthalpy and entropy. Spontaneity of reactions, free energy. Ionization of water. pH. Acidity and alkalinity. Strong and weak acids and bases, hydrolysis, buffers. The chemistry of carbon, the carbon valence. The functional groups. Alcohols and thiols. Redox reactions. Oxidation of alcohols and thiols. Aldehydes and ketones. Hemiacetals. Amines. Carboxylic acid. Amides, esters and anhydrides. Fatty acids. Triglycerides. Phospholipids. Biomolecules and their functional groups. Carbohydrates: Monosaccharides: glucose, fructose, ribose and deoxyribose, disaccharides: sucrose, and polysaccharides, cellulose, glycogen. Amino acids. Peptides and proteins. The peptide bond. protein structure. Biochemistry Myoglobin. Hemoglobin. Enzymes: structure and function. Catabolism and anabolism: general concepts of the intermediate metabolism. Glycolysis, gluconeogenesis and glucose: regulation. Lactate cycle. Krebs cycle. Strategies for ATP synthesis. Synthesis and degradation of glycogen. Oxidation of fatty acids. Chetonic bodies. Amino acids catabolism: urea cycle. RECOMMENDED READING/BIBLIOGRAPHY Catani, Savini, Guerrieri, Avigliano “Appunti di Biochimica per le lauree triennali” Editore: Piccin Stefani, Taddei “Chimica biochimica e biologia applicata” Editore: Zanichelli TEACHERS AND EXAM BOARD MONICA AVERNA Ricevimento: Students can meet the teachers by booking individual appointments. The teacher can be contacted by e-mail. e mail: monica.averna@unige.it Exam Board MONICA AVERNA (President) PIETRO BALDELLI MARIANNA DIONISI PIERLUIGI VALENTE MARIO PASSALACQUA (President and Coordinator of Integrated Course) LESSONS LESSONS START Consult detailed timetable at the following link: https://easyacademy.unige.it/portalestudenti/ Class schedule The timetable for this course is available here: Portale EasyAcademy EXAMS EXAM DESCRIPTION Oral examination Please refer to the specific AulaWeb application for any updates due to changes in the health and epidemiological situation. ASSESSMENT METHODS Oral examination with assessment of knowledge and ability to link between the various topics in the program. To establish the degree of competence acquired by the students, with respect to the program of this teaching, the commission will positively evaluate the ability to analyze and integrate different aspects of the proposed question and the use of an appropriate vocabulary. If the fundamental elements of the course are not known by the student, the exam will not be approved Agenda 2030 - Sustainable Development Goals Good health and well being
