CODE 66910 ACADEMIC YEAR 2024/2025 CREDITS 10 cfu anno 2 BIOTECNOLOGIE 8756 (L-2) - GENOVA TEACHING LOCATION GENOVA PREREQUISITES Propedeuticità in ingresso Per sostenere l'esame di questo insegnamento è necessario aver sostenuto i seguenti esami: Biotechnology 8756 (coorte 2023/2024) BIOLOGY II (CYTOLOGY, HISTOLOGY) AND LABORATORY COURSE 101966 2023 MODULES Questo insegnamento è composto da: CELLULAR BIOLOGY AND CELLULAR CULTURE LABORATORY DEVELOPMENTAL BIOLOGY TEACHING MATERIALS AULAWEB AIMS AND CONTENT LEARNING OUTCOMES Knowledge of the mechanisms that control cellular and molecular level the proliferation differentiation and cell death. Knowledge of the different stages of embryonic development of model organisms used in biotechnology research with particular attention to its control mechanisms. TEACHING METHODS Lectures and practical laboratory. Any Student with documented Specific Learning Disorders (SLD), or with any special needs, should contact the Lecturer(s) and to the dedicated SLD Representative in the Department before class begins, in order to liaise and arrange the specific teaching methods so that the learning aims and outcomes may be met. SYLLABUS/CONTENT Cell cycle and its regulation: Cell cycle, cycle control system S-phase, mitosis, cytokine, division control and cell growth Hayflick limit. Cell growth factors and proliferation Cell death: Apoptosis, proteins that regulate apoptosis Necrosis and autophagy Communication between cells: General aspects of cellular communication Signaling via enzyme-linked receptors Signaling via G-protein and intracellular mediators Ion channels. Signal transduction and cellular response. Intracellular signaling pathways Cell interactions: Adhesion proteins, cell-cell adhesion Basal plate, integrins and extracellular matrix Cell-ECM interactions Oncogenic transformation: Direct and indirect causes, microevolutionary process, tumour progression Proto-oncogenes, oncogenes, anti-oncogenes Oncogenesis from chemical, physical and viral agents Molecular diagnosis and new therapeutic approaches Stem cells: embryonic, foetal and adult, cancer stem cells Basic cell culture laboratory: establishment of animal and/or human cell cultures Preparation of culture media, thawing and freezing of cell lines or primary cultures. In vitro cell line expansion, cell count, viability test (MTT). Contamination of cell cultures. Cell biobanks. RECOMMENDED READING/BIBLIOGRAPHY Alberts B. et al. - Biologia Molecolare della cellula. Zanichelli. TEACHERS AND EXAM BOARD SIMONA CANDIANI Ricevimento: The students are able to book an appointment by phone, by mail or by aulaweb MATTEO BOZZO Ricevimento: By appointment, upon contacting the Lecturer by e-mail: matteo.bozzo@unige.it CHIARA GENTILI Ricevimento: By appointment by email: chiara.gentili@unige.it SARA TAVELLA Ricevimento: by appointment by email: sara.tavella@unige.it SVEVA BOLLINI Ricevimento: Prof. Sveva Bollini office hours for students are normally set by previous appointment requested by email. The office address is: Biology Section, Dept. Experimental Medicine; entrance: Via Pastore 3 or C.so Europa 30, second floor or Torre C3, c/o CBA, Cellular Oncology Lab, L. go R. Benzi 10, 16132 Genova. The lecturer's e-mail address and phone number are the following: sveva.bollini@unige.it; 0105558394. If necessary appointments may also take place on-line, depending on previous agreements between students and lecturer. ROBERTA TASSO Ricevimento: Prof. Roberta Tasso: The office address is: Biology Section, Dept. Experimental Medicine; entrance: Via Pastore 3 or C.so Europa 30, second floor. The teacher's e-mail address is the following: roberta.tasso@unige.it. Alternatively, the teacher can be reached at the following phone number: 0105558394. If necessary, appointments may also take place on-line, depending on previous agreements between the students and the teacher. Exam Board SIMONA CANDIANI (President) CHIARA GENTILI (President) MATTEO BOZZO LESSONS LESSONS START I semester, October. Class schedule CELLULAR BIOLOGY AND DEVELOPMENT AND CELLULAR CULTURE AND DEVELOPMENT LABORATORY EXAMS EXAM DESCRIPTION Oral. ASSESSMENT METHODS Oral examination.
