CODE 80808 ACADEMIC YEAR 2024/2025 CREDITS 4 cfu anno 3 BIOTECNOLOGIE 8756 (L-2) - GENOVA SCIENTIFIC DISCIPLINARY SECTOR CHIM/01 LANGUAGE Italian TEACHING LOCATION GENOVA SEMESTER 2° Semester TEACHING MATERIALS AULAWEB AIMS AND CONTENT LEARNING OUTCOMES The course provides an overview of the sampling procedures and instrumental analytical techniques used in biotechnology. Several aspects of the quality control will be treated also. TEACHING METHODS Frontal lessons. Any Student with documented Specific Learning Disorders (SLD), or with any special needs, shall reach out to the Lecturer(s) and to the dedicated SLD Representative in the Department before class begins, in order to liase and arrange the specific teaching methods and ensure proper achievement of the learning aims and outcomes. SYLLABUS/CONTENT Sample pre-treatment: physical and chimical methods. Criteria for the choice of an analytical method; figures of merit. Chromatographic techniques: TLC, high-performance liquid chromatography (HPLC) and gas chromatography (GC). Evaluation parameters in chromatography. Optimisation of a chromatographic method. Block schemes of instrumental equipment. Introduction to spectroscopic methods. Ultraviolet-visible (UV-VIS) spectrophotometry; qualitative and quantitative applications. Principles of fluorescence spectroscopy. Cytofluorimetry. Principles of infrared (IR) spectroscopy. Mass spectrometry (MS): principles and applications. Components of a mass spectrometer. Ionisation modes. Analysers. Basic principles for interpretation of mass spectra. Nuclear magnetic resonance (NMR) spectroscopy. Principles of the technique. Information provided by a 1H-NMR spectrum. Quality control. Good laboratory practices (GLP). Validation of an analytical method. Errors. Certified reference materials. Significant figures. Control charts. RECOMMENDED READING/BIBLIOGRAPHY Skoog - Leary, Chimica Analitica Strumentale, Edises. Material provided in class and/or available on Aulaweb. TEACHERS AND EXAM BOARD PAOLO OLIVERI Ricevimento: At the Pharmaceutical and Food Chemistry and Technologies Section of the Department of Pharmacy – DIFAR (Viale Cembrano, 4) or remotely by MS-Teams, upon appointment to be agreed via e-mail (paolo.oliveri@unige. it). Exam Board PAOLO OLIVERI (President) MONICA CASALE RICCARDO LEARDI CRISTINA MALEGORI LESSONS Class schedule ANALYTICAL INSTRUMENTATION AND CONTROL OF QUALITY EXAMS EXAM DESCRIPTION Oral. ASSESSMENT METHODS The course does not require midterm. The credits will be acquired by passing an oral examination. The oral examination is always conducted by two faculty members and has a duration of at least 30 minutes. With these modes, the commission is able to verify with high accuracy to achieve the educational objectives of teaching by putting different questions related to the program actually carried out during the course. When the educational objectives are not achieved, the student is invited to deepen his knowledge and to use any further explanation by the lecturer. Exam schedule Data appello Orario Luogo Degree type Note 15/01/2025 09:30 GENOVA Orale 05/02/2025 09:30 GENOVA Orale 18/06/2025 09:30 GENOVA Orale 11/07/2025 09:30 GENOVA Orale 18/07/2025 09:30 GENOVA Orale 01/09/2025 09:30 GENOVA Orale 26/09/2025 09:30 GENOVA Orale