CODE 75420 ACADEMIC YEAR 2024/2025 CREDITS 1 cfu anno 3 TECNICHE DI LABORATORIO BIOMEDICO 9293 (L/SNT3) - GENOVA LANGUAGE Italian TEACHING LOCATION GENOVA SEMESTER 1° Semester MODULES Questo insegnamento è un modulo di: PATHOLOGIC ANATOMY AND IMMUNOHISTOCHEMICAL (IHC) TECHNIQUES TEACHING MATERIALS AULAWEB OVERVIEW The course is aimed at presenting the main biomolecular typing techniques currently used in Pathological Anatomy mainly but not limited to Precision Medicine and individualized therapies. AIMS AND CONTENT LEARNING OUTCOMES At the end of the course the student will acquire knowledge on the main molecular markers of diagnosis, prognosis and therapeutic indication currently used in a Precision Medicine context. The student will also acquire skills on the main investigation methods for the aforementioned markers and on the organization of workflows in a molecular pathology laboratory AIMS AND LEARNING OUTCOMES The student will acquire expertise in the management of the molecular pathology laboratory connected to pathological anatomy, in the pre-analytical, analytical and post-analytical phases including quality controls on the main investigation systems and on the biological and clinical characteristics of the main markers PREREQUISITES Preliminary knowledge of molecular biology and pathological anatomy SYLLABUS/CONTENT Workflows in Pathological Anatomy Precision Medicine General information on ancillary methods in Pathological Anatomy The Mutations Structural organization of a molecular pathology laboratory The Microdissection The extraction of nucleic acids Storage of nucleic acids Southern Blots Northern Blots Western blot Other blotting methods PCR and Variants Classic sequencing Next Generation Sequencing The main molecular markers (ALK, BRAF, BRCA, EGFR, HER2/neu, TRAK, RAS et al) TEACHERS AND EXAM BOARD VALERIO GAETANO VELLONE Exam Board ROBERTO FRANCO FIOCCA (President) FEDERICA GRILLO (President) LUCA MASTRACCI (President) GIORGIA ANSELMI MARIANGELA LANATA VALERIO GAETANO VELLONE (President and Coordinator of Integrated Course) LESSONS Class schedule MOLECULAR BIOLOGIC TECHNIQUES IN PATHOLOGIC ANATOMY EXAMS Exam schedule Data appello Orario Luogo Degree type Note 13/01/2025 08:30 GENOVA Scritto + Orale 03/02/2025 08:30 GENOVA Scritto + Orale 24/02/2025 08:30 GENOVA Scritto + Orale 09/06/2025 08:30 GENOVA Scritto + Orale 07/07/2025 08:30 GENOVA Scritto + Orale 23/07/2025 08:30 GENOVA Scritto + Orale 15/09/2025 08:30 GENOVA Scritto + Orale