CODE 73160 ACADEMIC YEAR 2024/2025 CREDITS 2 cfu anno 3 TECNICHE DI LABORATORIO BIOMEDICO 9293 (L/SNT3) - GENOVA SCIENTIFIC DISCIPLINARY SECTOR MED/50 LANGUAGE Italian TEACHING LOCATION GENOVA SEMESTER 1° Semester MODULES Questo insegnamento è un modulo di: APPLIED MEDICAL SCIENCE AND CHROMATOGRAPHIC TECHNIQUES TEACHING MATERIALS AULAWEB AIMS AND CONTENT LEARNING OUTCOMES Understanding of the fundamental principles of flow cytometry and ability to apply it in its methodological choice. Sample preparation methods for flow cytometric analysis, coming from all possible tissues. Selection of appropriate controls, analysis of results and management of errors. Provide the ability to correctly use a flow cytometer analyzer or one capable of selecting cells (FACS sorter). Knowledge of the principles of Cell Sorting Provide the ability to set up panels and masks, even complex ones, for data acquisition and analysis for multiparametric flow cytometry in the context of diagnostic-clinical and/or transactional research projects Quality management systems in healthcare services and laboratories UNI EN ISO standards ISO, reference standards Clinical risk Error handling AIMS AND LEARNING OUTCOMES Discipline of: "Quality Management Systems" The course presents the main aspects of quality management within healthcare processes. Numerous examples are analyzed and presented, which allow identifying the main causes of deviation from the expected quality. The educational objectives for the student are: knowledge, understanding and application of quality control methodologies knowledge of the rules governing quality control and management in the health sector. Discipline of: "Cytofluorimetry techniques" Attendance and active participation in training activities (in the classroom and, when possible, in the laboratory) will allow students to: know the basic parts of a flow cytometer know how to use a flow cytometer knowing how to choose the best performing reagents on the machine that will be used be able to understand and build a diagnostic or applied research panel of multiparametric flow cytometric analysis know how to solve cytometric problems be able to do analysis on an acquired cytometric file TEACHING METHODS Frontal lessons in the classroom with video projections of the teacher's slides or, in the event of a health emergency, carried out remotely. The lessons aim at the constant involvement of the students and at the technical-scientific debate SYLLABUS/CONTENT Integrated course of "Applied Medical Technical Sciences" Discipline of: "Quality Management Systems" - Lecturer: Nidia Sofia Diaz Gaitan Evolution of Quality The leadership Customer orientation Understand and improve Processes The Deming Cycle Teamwork The Ligurian model governing the accreditation of healthcare facilities Institutional accreditation The Manual of the Liguria Region Accreditation and Quality Requirements ISO organizational models Reference standards UNI EN ISO 9001:2015 standard Quality Management System Audits How to conduct an Audit Dynamic systems for process improvement Verification of the effectiveness of corrective actions Elements of Clinical Risk Management The error human behavior Error Classification and Management Clinical risk management tools: Clinical Audits, Incident Reporting, F.M.E.A. and R.C.A. Discipline of: "Cytofluorimetry techniques" - Lecturer: Daniele Reverberi Flow cytometry: Introduction, meaning and recommended bibliography Brief history of technology Fluorescence and fluorochromes: principles and functioning, general notions on dyes for nucleic acids and proteins The fundamental parts of the machine and the theoretical principles applied: fluidics - principles of hydrodynamics applied; optics - light, theory and materials; electronics - data acquisition, analysis and representation. Instrument performance procedures: instrument control and analysis threshold setting Sample preparation: basic knowledge for sample preparation; treatment and analysis of samples from solid tissues. Multiparametric flow cytometry: panel preparation and setting of antibodies and fluorochromes Problem solving: spill-over and compensation, autofluorescence, quenching and bleaching Basic management and analysis of the generated data: main concepts connected with the analysis of a cytometric file: notions on the different gating methods; off-line fluorescence compensation; Optimization of necessary controls: isotype control, fluorescence minus one (FMO) and blank; criteria for evaluating positivity; main types of representation of cytometric data. Flow Cell Sorting: theory and instrumental functioning, examples of applications Applications of the cytometric platform in biomedical research and diagnostics: evaluation of the cell cycle and cell proliferation; evaluation of apoptosis, necrosis and cell death (outline) Microparticles in cytofluorimetry; I mention the latest generation tools RECOMMENDED READING/BIBLIOGRAPHY Discipline of: "Quality Management Systems" Manual for Institutional Accreditation of Healthcare Activities of the Liguria Region, pursuant to Presidential Decree 14 January 1997 and of the L.R. July 30, 1999 No. 20. Decree of the President of the Republic 14/01/1997 (publ. on the ordinary supplement to the Official Gazette n. 42 of 20 February 1997). Approval of the guidance and coordination act for the regions and autonomous provinces of Trento and Bolzano, regarding the minimum structural, technological and organizational requirements for the exercise of healthcare activities by public and private structures. Joint Commission, Introduction to the Principles of Quality Improvement ISO UNI EN 9001:2015 standard Novaco F., Damen V., Clinical risk management, Centro Scientifico Editore Rasmussen J, Duncan K. & Leplat J., New Technology and human error. 1987 Reason J., Human error; Cambridge University Press. Italian edition: Human error; Publisher Il Mulino, Bologna BMJ 2000 Discipline of: "Cytofluorimetry techniques" Shapiro H., "Practical Flow Cytometry", Wiley-Liss, New York, 4th Edition Longobardi Givan A. “Flow Cytometry, first principles” Bushnell T. and Kissner B.A. "Advanced topics in 4-10 color compensation" Cytometry (PubMed - US National Library of Medicine National Institute of Health): Part A : the journal of the International Society for Analytical Cytology Part B : Clinical cytometry European Journal of Immunology (PubMed - US National Library of Medicine National Institute of Health) TEACHERS AND EXAM BOARD NIDIA SOFIA DIAZ GAITAN DANIELE REVERBERI Ricevimento: Teachers receive students upon notice to the following email addresses: nidiasofia.diazgaitan@edu.unige.it daniele.reverberi@hsanmartino.it IRCCS San Martino Polyclinic Hospital Exam Board DANIELE REVERBERI (President) LAURA BARBAGALLO NIDIA SOFIA DIAZ GAITAN MADDALENA MASTROGIACOMO (President and Coordinator of Integrated Course) LESSONS LESSONS START III year, I semester Class schedule APPLIED TECHNICAL MEDICAL SCIENCES EXAMS EXAM DESCRIPTION The exam is exclusively oral for both disciplines of the module; it is possible to request the solution of analysis or design schemes presented to the student on paper, but always at the time of the oral exam. The oral exam will therefore consist of 4 or 5 general questions which will be explored during the test until the teacher obtains a sufficient analysis of the student's preparation. Extraordinary exam sessions will not be granted outside the periods and dates indicated in the study program regulations, with the exception of extraordinary sessions for students outside the course after a request has been examined by all the professors of the integrated course. ASSESSMENT METHODS Discipline of: "Quality Management Systems" The oral exam will mainly focus on the topics covered during the frontal lessons and on the material uploaded on aulaweb. The aim is to evaluate if the student has reached an adequate level of knowledge and if he has acquired the ability to apply the concepts to clinical practice using the appropriate terminology. Discipline of "Cytofluorimetry techniques" The oral exam will mainly focus on the topics covered during the lectures and on any visits to the laboratories and will aim to evaluate whether the student has reached an adequate level of knowledge and whether he has acquired the ability to critically analyze problems related to a survey diagnostic or research flow cytometry. The student may also be asked to build a simple analysis panel, identifying some simple cell subtypes. The ability to describe processes clearly and with correct terminology will be positively evaluated. Exam schedule Data appello Orario Luogo Degree type Note 10/01/2025 14:30 GENOVA Orale aula 7 polo alberti 04/02/2025 14:30 GENOVA Orale atleta di patologia generale NB l'esame è anticipato alle ore 13 21/02/2025 14:30 GENOVA Orale esame alle 13:30 nell'aula ex patologia 13/06/2025 14:30 GENOVA Orale 07/07/2025 14:30 GENOVA Orale 21/07/2025 14:30 GENOVA Orale 05/09/2025 14:30 GENOVA Orale Agenda 2030 - Sustainable Development Goals Good health and well being Quality education Decent work and economic growth