AIMS AND CONTENT

LEARNING OUTCOMES

Chromatographic techniques and principles of mass spectrometry. Gas liquid chromatography – mass spectrometry. Validation of a chromatographic method
Application of chromatographic methods in the biomedical laboratory. Understanding chromatographic separation. Know how to evaluate chromatographic resolution. Know how to distinguish the various types of chromatographic techniques
Understanding the use of the HPLC technique. understand the choice and use of various detection systems in chromatography. Acquisition of the concept of external standard and internal standard
Provide the ability to apply the chromatographic technique. Provide the relative theoretical bases of chemical-physical of fluorochromes and their interaction with light (principles of Optics). Ability to choose and use the correct fluorochrome and its application in multiparametric flow cytometry.

AIMS AND LEARNING OUTCOMES

The Chromatography module proposes the study of the fundamental principles of the technique and of the detection systems. The attention is focused on Mass Spectrometry, Gas Chromatography, techniques widely used in work activities. The basic principles provided will facilitate the internships by introducing the student to the working world.

In particular:

Provide the theoretical foundations related to Chromatography and Chromatographic Techniques and Detection Systems
To acquire the fundamental principles of Mass Spectrometry, Gas Chromatography and Gas Chromatography-Mass Spectrometry, Liquid Chromatography-Mass Spectrometry
To make known the lines of the Validation Procedure of a chromatographic method
Know the main applications of chromatographic methods in the biomedical laboratory
Provide the ability to perform HPLC chromatographic analysis methods

Therefore the student will have to acquire the basic concepts of the functioning of chromatographic techniques in order to be able to autonomously evaluate how to apply them to assist in the clinical diagnosis of specific pathologies

TEACHING METHODS

Frontal lessons in the classroom with projected slide presentations and simulation of practical applications.

SYLLABUS/CONTENT

Introduction to Chromatography

Matter and substances

States of aggregation of matter and changes of state

Classification of chromatographic techniques

Gas chromatography and detectors

HPLC and detectors

Gas chromatography-mass spectrometry

Liquid chromatography-mass spectrometry

Quantitative methods in chromatography

Validation of a chromatographic method

Applications of chromatographic methods in the biomedical laboratory

RECOMMENDED READING/BIBLIOGRAPHY

We propose the study of what is reported in the slides presented and discussed.

TEACHERS AND EXAM BOARD

Exam Board

DANIELE REVERBERI (President)

LAURA BARBAGALLO

NIDIA SOFIA DIAZ GAITAN

MADDALENA MASTROGIACOMO (President and Coordinator of Integrated Course)

LESSONS

Class schedule

CHROMATOGRAPHIC TECHNIQUES

EXAMS

EXAM DESCRIPTION

The exam can take place in oral form through discussion of all the topics covered or in written form as a multiple choice test

ASSESSMENT METHODS

The evaluation of the student will focus on the assessment of the knowledge transmitted through the lessons and on the ability to link the application of the techniques to the diagnosis of pathologies