CODE 104225 ACADEMIC YEAR 2024/2025 CREDITS 6 cfu anno 1 BIOLOGIA APPLICATA E SPERIMENTALE 11158 (LM-6) - GENOVA SCIENTIFIC DISCIPLINARY SECTOR BIO/06 TEACHING LOCATION GENOVA SEMESTER 2° Semester TEACHING MATERIALS AULAWEB OVERVIEW The Forensic Cytology and Histology course offers in-depth training on the histological and microscopic characteristics of tissues and bodily fluids with forensic applications. Students will learn to distinguish, identify, and detect signs of cellular and tissue alterations due to pre- and post-mortem phenomena. Methods for recognizing and characterizing biological samples will be examined, evaluating the most suitable techniques for their processing. The course includes the analysis of histological indicators and the assessment of laboratory technique quality. Students will develop skills to propose, structure, and combine plans for cytological and histological analyses, and present results with scientific clarity. The acquired training will enable students to monitor and verify the importance of microscopic data in reconstructing forensic events, effectively supporting legal investigations. AIMS AND CONTENT LEARNING OUTCOMES The forensic cytology and histology focus on body fluids and tissues of interest in forensic science including blood, menstrual blood, semen, vaginal material, saliva, skin and their derivates (hairs and nails). The course aims to provide key competences on presumptive and confirmatory tests used in the identification of the body fluids. The structure and organization of hairs, scales, teeth from different species but with special focus on human, as well as the histology of bone tissue and of forensically relevant soft tissues, will be illustrated. The students will be able to acquire the main cytodiagnostic techniques (immunohistochemistry, in situ hybridization, light and electron microscopy, etc.) that can be used to identify the various types of physical injuries or trauma. Some case reports and in-depth analysis on specific topics (e.g. neurotraumatology and forensic aspects of fetal deaths) will be also reported. The course is intended to endow the student with useful knowledge and methods in the event reconstruction in investigations and in providing microscopic confirmation of macroscopic findings, facilitating a more comprehensive reporting. The approach of the course will allow the student to apply the knowledge to a wide range of forensic cases, from violent crime to fraud. AIMS AND LEARNING OUTCOMES Attendance in training activities and individual study will enable the student to: Describe in detail, using appropriate terminology, the histological characteristics of a selection of forensic tissues, predominantly human, comparing them with those of other animals. Describe in detail, using appropriate terminology, the microscopic characteristics of various bodily fluids, mainly human, comparing them with those of other animals. Illustrate histological indicators of cellular and tissue alterations due to pre- and post-mortem phenomena. Describe the main methods for recognizing and characterizing tissues or bodily fluids found in the environment. Evaluate the most suitable techniques for their processing. Describe the main methods for treating cytological and histological samples. Evaluate the most suitable techniques for their processing. Analyze, distinguish, and identify the microscopic and macroscopic characteristics of the examined samples. Detect and determine cellular and tissue alterations due to pre- and post-mortem phenomena. Propose and develop innovative methods for the recognition and characterization of cytological and histological samples. Monitor and verify the quality of sample processing techniques. Structure, combine, and design plans for histological and cytological analyses for specific forensic contexts. Present and judge the analysis results clearly and in detail, using appropriate scientific terminology. Appreciate and evaluate the importance of microscopic data in the reconstruction of forensic events PREREQUISITES Knowledge in basic cytology, histology, microscopy, and histological techniques. TEACHING METHODS The course consists of lectures, seminars by experts in forensic odontology and reproductive biology (forensic aspects of seminal fluid and vaginal secretions), and seminar activities organized by students. Students who have a valid certification of physical or learning disabilities deposited at the University and who wish to request compensatory tools, dispensatory measures, or other accommodations for lectures, assignments, and exams should speak with both the instructor and Professor Sara Ferrando (sara.ferrando@unige.it), the contact person at the Department for Disabilities. SYLLABUS/CONTENT The role of cytology and histology in forensic analysis Pre- and post-mortem variables affecting forensic cytology and histology Microscopic changes post-mortem The role of microscopy in forensic analysis Forensic serology: presumptive and confirmatory tests Blood: general aspects and forensic implications Seminal fluid: general aspects and forensic implications Vaginal secretions: general aspects and forensic implications Urine: general aspects and forensic implications Sweat: general aspects and forensic implications Fecal material: general aspects and forensic implications Saliva: general aspects and forensic implications Vomitus and hematemesis: general aspects and forensic implications Histological techniques: forensic aspects Artifacts: learning to recognize and correct common errors Soft tissues: influence of pre- and post-mortem events on histological treatment and data interpretation Bone tissue (general): influence of pre- and post-mortem events on histological treatment and data interpretation Bone tissue (tooth): influence of pre- and post-mortem events on histological treatment and data interpretation Skin appendages: structure of hair and hair follicles. Microscopic investigations to study hair degradation. Identification of hairs from different mammal species. Other skin appendages: an overview of nail structure and forensic implications. During the course, 2 hours of preparatory lessons will be dedicated to the voluntary presentation of a topic related to the course. The lesson will include an in-depth exploration of how to write/read a scientific article in relation to the GIMBE, ARRIVE, CONSORT, CARE, and PRISMA guidelines RECOMMENDED READING/BIBLIOGRAPHY Slides of the lessons, scientific articles and other teaching material will be provided on Aulaweb TEACHERS AND EXAM BOARD SIMONA CANDIANI Ricevimento: The students are able to book an appointment by phone, by mail or by aulaweb ANDREA AMAROLI Exam Board ANDREA AMAROLI (President) SARA FERRANDO (President) STEFANO AICARDI MATTEO BOZZO SIMONA CANDIANI TIZIANA BACHETTI (Substitute) LESSONS LESSONS START The lessons timetable and academic calendar will be available on the dedicated website Class schedule FORENSIC CYTOLOGY AND HISTOLOGY EXAMS EXAM DESCRIPTION Oral assessment + presentation of a case of study. Three exam dates will be available in the winter session (January-February) and four in the summer session (June, July, September). In case of certification of specific learning disability, disability or other special educational needs, the University legislation and regulations on disability and SLD are applied (https://unige.it/disabilita-dsa/studenti-disabilit%C3% A0-normative). See also the "Other information" section of this syllabus. ASSESSMENT METHODS The oral exam regards the topics seen during the lessons. Questions can be posed verbally or by asking to describe histologic and microscopic photographs. The ability of the student in indicating the most suitable methods to analyze different samples of forensic interest will be also evaluated. Furthermore, the use of correct terminology will be considered. The level of detail required for each topic is clarified during the lessons. The preparation and presentation of a case of study are requested in order to evaluate the interpretive ability of the student regarding the scientific literature regarding the exam topic. Exam schedule Data appello Orario Luogo Degree type Note 16/01/2025 09:30 GENOVA Orale 05/02/2025 09:30 GENOVA Orale 17/02/2025 09:30 GENOVA Orale 12/06/2025 09:30 GENOVA Orale 27/06/2025 09:30 GENOVA Orale 18/07/2025 09:30 GENOVA Orale 18/09/2025 09:30 GENOVA Orale FURTHER INFORMATION In case of certification of specific learning disability, disability or other special educational needs, please contact both the Department contact person, Prof. Ferrando Sara, and the lecturer at the beginning of the lectures to agree on teaching and exam methods which, in compliance of the teaching objectives, take into account individual learning methods and provide suitable compensatory/dispensatory tools recognized by the University SLD Student Service.