CODE 62739 ACADEMIC YEAR 2022/2023 CREDITS 6 cfu anno 2 SCIENZA E INGEGNERIA DEI MATERIALI 9017 (LM-53) - GENOVA 6 cfu anno 1 SCIENZA E TECNOLOGIA DEI MATERIALI 11430 (LM SC.MAT.) - GENOVA 6 cfu anno 1 FISICA 9012 (LM-17) - GENOVA 6 cfu anno 2 FISICA 9012 (LM-17) - GENOVA SCIENTIFIC DISCIPLINARY SECTOR FIS/07 TEACHING LOCATION GENOVA SEMESTER 1° Semester PREREQUISITES Propedeuticità in ingresso Per sostenere l'esame di questo insegnamento è necessario aver sostenuto i seguenti esami: PHYSICS 9012 (coorte 2021/2022) MATTER PHYSICS 2 61844 2021 THEORETICAL PHYSICS 61842 2021 NUCLEAR AND PARTICLE PHYSICS AND ASTROPHYSICS 2 61847 2021 TEACHING MATERIALS AULAWEB OVERVIEW The Biophysics laboratory allows students to learn the most modern experimental methods to investigate biological samples, working directly on research equipment supplied by the Biophysics group operating within the Department of Physics. AIMS AND CONTENT LEARNING OUTCOMES The course teaches some techniques for the study of the physical properties of biological systems. AIMS AND LEARNING OUTCOMES The student will learn how to deal with complex biophysical problems, such as the self-assembly of biomolecules, the folding and unfolding of proteins and the transport processes through the cell membrane. The investigation will carried out at the molecular or single cell level. At the end of the course the student will be able to: - understand which experimental methods are best suited to study a particular biophysical process - apply the acquired knowledge to design a biophysical experiment - acquire the skills to conduct an experiment in the laboratory - critically analyze the results of an experiment The Biophysics Laboratory teaching can be chosen by students enrolled in the Master's Degree Course in Materials Science and Technology with particular reference to the "Materials Scientist: Research Specialist" profile. PREREQUISITES The course can be followed with profit by students who have obtained a three-year degree in a scientific field. TEACHING METHODS The course includes both lectures and laboratory experiences. Lectures are preparatory to the performance of laboratory experiences. For each laboratory experience, students are required to write a scientific report. Each report must contain an introductory part describing the state of the art, a part with the results obtained and a critical discussion of the results. SYLLABUS/CONTENT Scanning Tunneling Microscopy (STM): operating principle, scanning system, operation mode (constant current/height), resolution Atomic Force Microscopy (AFM): operating principle, scanning system, operation mode (contact/tapping), resolution, force spectroscopy, nanolithography (nanoshaving and nanografting) Optical microscopy: fluorescence, confocal and two-photon microscopy, operating principles, fluorescent probes, photobleaching, super-resolution techniques Patch clamp technique for electrophysiological measurements: seal test, main configurations (whole cell, inside-out, outside-out, perforated patch), analysis of ionic currents Protein crystallography: protein crystallization methods, outline of the methods used to determine the 3D structure of proteins Laboratory experiences focus on the topics covered in the lectures. In particular: - atomic force microscopy for the study of protein structures and thin organic films - single-cell fluorescence microscopy for the study of intracellular calcium - confocal and super-resolution microscopy - patch clamp technique for the study of ionic currents across the cell membrane - protein crystallization and crystal observation / manipulation RECOMMENDED READING/BIBLIOGRAPHY All the necessary material will be provided on Aulaweb TEACHERS AND EXAM BOARD ORNELLA CAVALLERI Ricevimento: On request ALBERTO GIOVANNI DIASPRO ALESSANDRA PESCE Ricevimento: On request ELENA GATTA Exam Board ALBERTO GIOVANNI DIASPRO (President) ELENA GATTA ALESSANDRA PESCE ORNELLA CAVALLERI (President Substitute) LESSONS LESSONS START The course takes place in the first semester. Class schedule LAB OF BIOPHYSICS EXAMS EXAM DESCRIPTION The exam consists of an oral test during which the student presents, at his or her choice, one of the experiences carried out in the laboratory and answers questions asked by the jury relating to the other topics of the course and to the reports describing the laboratory experiences. ASSESSMENT METHODS The verification of learning will take place through the drafting of a scientific report for each experience conducted in the laboratory. Each report must contain an introductory part describing the state of the art, a part with the results obtained and finally a critical discussion of the results. Exam schedule Data Ora Luogo Degree type Note 25/01/2023 09:00 GENOVA Orale 15/02/2023 09:00 GENOVA Orale 20/06/2023 09:00 GENOVA Orale 19/07/2023 09:00 GENOVA Orale 12/09/2023 09:00 GENOVA Orale